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Catalog Number: (100243-842)

Supplier: Southern Biotechnology

Description: The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells while allowing them to attack infected or transformed cells in which class I molecules have been downregulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains ~11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice.

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Catalog Number: (100243-022)

Supplier: Southern Biotechnology

Description: CD106, also known as VCAM-1, is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (e.g., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The M/K-2 monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under non-reducing conditions.

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Catalog Number: (100245-070)

Supplier: Southern Biotechnology

Description: Rabbit F(ab')2 Anti-Mouse IgG(H+L)-HRP antibody for use in ELISA and immunocytochemistry assays.

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Catalog Number: (100244-376)

Supplier: Southern Biotechnology

Description: Goat Anti-Human Lambda-AP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

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Catalog Number: (100245-104)

Supplier: Southern Biotechnology

Description: Rabbit Anti-Human IgG(H+L)-HRP antibody for use in ELISA, immunocytochemistry, and western blotting assays.

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Catalog Number: (100243-514)

Supplier: Southern Biotechnology

Description: CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.

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Supplier: Southern Biotechnology

Description: Goat Anti-Mouse IgG2a-APC antibody with minimal reactivity to human proteins for use in flow cytometry assays.

Catalog Number: (100242-028)

Supplier: Southern Biotechnology

Description: Purified Goat F(ab’)2 IgG for use as a control in flow cytometry and immunocytochemistry assays and as a standard in ELISA.

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Catalog Number: (100245-178)

Supplier: Southern Biotechnology

Description: Rabbit Anti-Mouse IgG(H+L)-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunocytochemistry, and western blotting assays.

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Catalog Number: (103259-844)

Supplier: Southern Biotechnology

Description: Alexa Fluor 488 Anti-Mouse IgG2a antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

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Catalog Number: (100245-468)

Supplier: Southern Biotechnology

Description: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on antigen presenting cells. Peripheral γδ+ T cells are CD28 negative. Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A, or monoclonal antibodies against the avian T cell receptor (TCR). Both Vβ1- and Vβ2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A but inhibited by herbimycin A suggesting that CD28 employs a signaling pathway at least partly distinct from that triggered by TCR/CD3. The monoclonal antibody AV7 also reacts with turkey CD28 as demonstrated by flow cytometry.

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Catalog Number: (100242-342)

Supplier: Southern Biotechnology

Description: Purified Anti-Human IL-7 antibody for use in ELISA and immunocytochemistry assays.

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Catalog Number: (100242-874)

Supplier: Southern Biotechnology

Description: HRP Anti-Mouse Lambda antibody for use in ELISA, immunohistochemistry, and western blotting assays.

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Catalog Number: (101442-542)

Supplier: Southern Biotechnology

Description: Rat IgG2a-PE/Texas Red for use as a control in flow cytometry assays.

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Catalog Number: (100245-456)

Supplier: Southern Biotechnology

Description: The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vβ families, Vβ1 and Vβ2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- subpopulations. The TCR-3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR-3+] cells in the blood and spleen express relatively high levels of the TCR-3/CD3 receptor complex and are “single positive” with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (~ 80% CD4+ vs ~ 20% CD8+).

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Catalog Number: (103265-042)

Supplier: Southern Biotechnology

Description: Purified Donkey F(ab')2 Anti-Mouse IgG(H+L) antibody for use in ELISA assays.

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