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Catalog Number: (100246-284)
Supplier: Southern Biotechnology
Description: CD45RA is a 220 kDa exon A-dependent isoform of CD45. It is expressed on 40-50% of CD4+ peripheral T cells, 50% of peripheral CD8+ T cells, and a portion of B lymphocytes and monocytes.


Catalog Number: (100243-794)
Supplier: Southern Biotechnology
Description: The CD45 antigen is an essential regulator of leukocyte activation and development and is now known to be a protein tyrosine phosphatase, e.g., CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling, and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685, and 1820) and as allelic forms CD45.1 (Ly-5.1) and CD45.2 (Ly-5.2). The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD45.1 allotype.


Catalog Number: (100243-204)
Supplier: Southern Biotechnology
Description: CD11b (integrin αM subunit) combines with CD18 (integrin β2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.


Catalog Number: (100245-800)
Supplier: Southern Biotechnology
Description: FITC Anti-Human IgG4 pFc' antibody for use in flow cytometry, immunohistochemistry, and western blotting assays.


Catalog Number: (100245-726)
Supplier: Southern Biotechnology
Description: HRP Anti-Human IgG2 Fc antibody for use in ELISA, immunocytochemistry, western blotting, and ELISpot assays.


Catalog Number: (100243-076)
Supplier: Southern Biotechnology
Description: CD3ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex of mouse strains tested.


Catalog Number: (100244-168)
Supplier: Southern Biotechnology
Description: CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. CD107b is similar in structure to Mac-1 and is expressed primarily on epithelial cells and macrophages in a variety of organs in normal and beige mutant mice.


Catalog Number: (103265-578)
Supplier: Southern Biotechnology
Description: Goat Anti-Mouse Kappa-Alexa Fluor 555 antibody for use in immunohistochemistry / immunocytochemistry and western blotting assays.


Catalog Number: (103264-736)
Supplier: Southern Biotechnology
Description: Goat Anti-Human IgG(H+L)-Alexa Fluor 555 antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in fluorescent assays.


Catalog Number: (103259-900)
Supplier: Southern Biotechnology
Description: CD11b, the integrin αM subunit, combines with CD18, the integrin β2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.


Catalog Number: (103264-822)
Supplier: Southern Biotechnology
Description: Goat Anti-Rat IgG-Alexa Fluor 555 antibody with minimal reactivity to mouse proteins for use in immunohistochemistry / immunocytochemistry and western blotting assays.


Catalog Number: (100244-224)
Supplier: Southern Biotechnology
Description: Goat Anti-Human IgM-PE antibody for use in flow cytometry and multiplex assays.


Catalog Number: (100244-220)
Supplier: Southern Biotechnology
Description: Goat Anti-Human IgM-Texas Red antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.


Catalog Number: (100242-606)
Supplier: Southern Biotechnology
Description: Goat Anti-Mouse Lambda-HRP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.


Catalog Number: (100243-190)
Supplier: Southern Biotechnology
Description: In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.


Catalog Number: (101442-824)
Supplier: Southern Biotechnology
Description: CY5 Anti-Rat IgG1 antibody for use in flow cytometry and immunohistochemistry assays.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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