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Catalog Number: (100245-494)
Supplier: Southern Biotechnology
Description: In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCR-γδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCR-γδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody EP42 recognizes the CD8β chain.


Catalog Number: (103259-996)
Supplier: Southern Biotechnology
Description: Goat Anti-Human Kappa-Alexa Fluor 647 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (100245-644)
Supplier: Southern Biotechnology
Description: HRP Anti-Human IgM antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (100243-174)
Supplier: Southern Biotechnology
Description: In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.


Catalog Number: (103259-754)
Supplier: Southern Biotechnology
Description: Purified Human IgG3 Lambda for use as a standard in ELISA.


Catalog Number: (100243-218)
Supplier: Southern Biotechnology
Description: CD11b (integrin αM subunit) combines with CD18 (integrin β2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.


Catalog Number: (103260-044)
Supplier: Southern Biotechnology
Description: Goat Anti-Human IgG Fc-AP antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.


Catalog Number: (100244-028)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 25-9-3 reacts specifically with the I-Ab haplotype of MHC Class II molecules. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.


Catalog Number: (100244-796)
Supplier: Southern Biotechnology
Description: Goat F(ab')2 Anti-Rabbit IgG(H+L)-Biotin antibody with minimal reactivity to mouse and human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (100241-972)
Supplier: Southern Biotechnology
Description: Mouse IgA-Biotin for use as a control in flow cytometry assays.


Catalog Number: (103265-274)
Supplier: Southern Biotechnology
Description: CD61 is the integrin β3 subunit, a 110 kDa transmembrane glycoprotein expressed on platelets, megakaryocytes, and endothelial cells. Integrin β3 associates with gpIIb (CD41) to form the CD41/CD61 complex that mediates platelet adhesion and aggregation. CD61 also associates with CD51 to form the αVβ3 vitronectin receptor.


Catalog Number: (103265-018)
Supplier: Southern Biotechnology
Description: Rabbit Anti-Mouse IgG(H+L)-Alexa Fluor 488 antibody for use in immunohistochemistry and western blotting assays.


Catalog Number: (103265-654)
Supplier: Southern Biotechnology
Description: Goat Anti-Human IgG Fc-AP antibody for use in ELISA and western blotting assays.


Catalog Number: (103259-798)
Supplier: Southern Biotechnology
Description: Goat Anti-Mouse IgG2a-Biotin antibody with minimal reactivity to human, bovine, and horse serum proteins for use in ELISA assays.


Catalog Number: (100242-364)
Supplier: Southern Biotechnology
Description: Goat Anti-Mouse IgM-FITC antibody with minimal reactivity to human proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (101442-772)
Supplier: Southern Biotechnology
Description: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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