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Catalog Number: (101442-790)
Supplier: Southern Biotechnology
Description: NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL/6, NZB, and CE). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.


Catalog Number: (100246-332)
Supplier: Southern Biotechnology
Description: CD55, also known as decay accelerating factor (DAF), is an 80 kDa GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters.


Catalog Number: (103265-246)
Supplier: Southern Biotechnology
Description: Alexa Fluor 555 Anti-Human Kappa antibody for use in fluorescent assays.


Catalog Number: (103264-608)
Supplier: Southern Biotechnology
Description: Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with the monoclonal antibody D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.


Catalog Number: (100245-394)
Supplier: Southern Biotechnology
Description: Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II transmembrane glycoprotein that is expressed as a heterodimer on the suppressor/cytotoxic subpopulation of peripheral T lymphocytes. It is present on approximately 76% of thymocytes, 14% of splenocytes, 1% of bone marrow cells, and 15% of peripheral blood lymphocytes. CD8 functions as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition.


Catalog Number: (103259-858)
Supplier: Southern Biotechnology
Description: Biotin Anti-Mouse IgG2b antibody for use in ELISA, flow cytometry, immunocytochemistry, and western blotting assays.


Catalog Number: (103259-712)
Supplier: Southern Biotechnology
Description: Rat IgG2b-Alexa Fluor 700 for use as a control in flow cytometry assays.


Catalog Number: (101442-842)
Supplier: Southern Biotechnology
Description: Purified Anti-Rabbit Light Chain antibody for use in ELISA and western blotting assays.


Catalog Number: (101190-282)
Supplier: Southern Biotechnology
Description: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.


Catalog Number: (103265-044)
Supplier: Southern Biotechnology
Description: Donkey F(ab')2 Anti-Mouse IgG(H+L)-FITC antibody for use in fluorescent assays.


Catalog Number: (100244-078)
Supplier: Southern Biotechnology
Description: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the α chain which binds IL-2 with low affinity; (ii) the β chain that binds IL-2 with high affinity; and (iii) the common γ chain (γc) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a Kd of 1.3 x 10-11 M. In mouse spleen, CD122 is expresed on ~30% of CD8+ cells and all NK cells but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R β chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. The monoclonal antibody 5H4 does not block IL-2 binding.


Catalog Number: (100245-916)
Supplier: Southern Biotechnology
Description: CD20 is a 33-37 kDa unglycosylated phosphoprotein. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. It is expressed on all stages of B cell differentiation except plasma cells. The CD20 molecule is involved in regulation of B cell differentiation presumably via its reported function as a Ca++ channel subunit.


Catalog Number: (100242-362)
Supplier: Southern Biotechnology
Description: Purified Goat Anti-Mouse IgM antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, immunoprecipitation, ELISpot, purification, and stimulation assays.


Catalog Number: (103265-092)
Supplier: Southern Biotechnology
Description: Donkey F(ab')2 Anti-Rat IgG(H+L)-HRP antibody with minimal reactivity to mouse serum proteins for use in ELISA assays.


Catalog Number: (103259-744)
Supplier: Southern Biotechnology
Description: Hamster IgG-Alexa Fluor 647 for use as a control in flow cytometry assays.


Catalog Number: (100244-248)
Supplier: Southern Biotechnology
Description: Goat Anti-Human IgD-Biotin antibody for use in ELISA, flow cytometry, immunohistochemistry, and separation assays.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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