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Catalog Number: (100242-520)
Supplier: Southern Biotechnology
Description: Purified Goat F(ab')2 Anti-Mouse IgG(H+L) antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.


Catalog Number: (100243-518)
Supplier: Southern Biotechnology
Description: CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.


Catalog Number: (103264-816)
Supplier: Southern Biotechnology
Description: Goat Anti-Rat IgG-β-galactosidase antibody with minimal reactivity to mouse proteins for use in ELISA and immunohistochemistry / immunocytochemistry assays.


Catalog Number: (103265-702)
Supplier: Southern Biotechnology
Description: CD19 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed at all stages of B cell differentiation except terminally differentiated plasma cells. It is also present on the cell surface of follicular dendritic cells. CD19 associates with CD21, CD81, Leu 13 and/or MHC Class II molecules to form a signal transduction complex on the B cell surface. This signaling complex modulates the activation threshold for the B cell antigen receptor (BCR).


Catalog Number: (101190-232)
Supplier: Southern Biotechnology
Description: Low Endotoxin/Azide Free Anti-Mouse IL-10 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, ELISA, ELISpot, neutralization assays.


Catalog Number: (103265-490)
Supplier: Southern Biotechnology
Description: Alexa Fluor 647 Anti-Mouse IL-2 antibody for use in flow cytometry and immunohistochemistry assays.


Catalog Number: (100245-688)
Supplier: Southern Biotechnology
Description: Biotin Anti-Human IgG Fc antibody for use in ELISA, flow cytometry, immunohistochemistry, western blotting, and multiplex assays.


Catalog Number: (103264-958)
Supplier: Southern Biotechnology
Description: Goat Anti-Chicken IgY(H+L)-Alexa Fluor 488 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (100245-200)
Supplier: Southern Biotechnology
Description: Rabbit Anti-Rat IgG(H+L)-Biotin antibody with minimal reactivity to human proteins for use in ELISA and immunohistochemistry assays.


Catalog Number: (100245-118)
Supplier: Southern Biotechnology
Description: Purified Rabbit Anti-Sheep IgG(H+L) antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.


Supplier: Southern Biotechnology
Description: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 (SB Cat. No. 1600). While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antibody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.

Supplier: Southern Biotechnology
Description: The monoclonal antibody DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and α2 integrin. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes. NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.

Catalog Number: (100241-996)
Supplier: Southern Biotechnology
Description: Mouse IgG-PE/CY5 (SPRD) for use as a control in flow cytometry assays.


Catalog Number: (100245-460)
Supplier: Southern Biotechnology
Description: CD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 10% of spleen cells, and 45% of peripheral blood lymphocytes but less than 1% of bursal cells.


Catalog Number: (100244-026)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 25-9-3 reacts specifically with the I-Ab haplotype of MHC Class II molecules. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.


Catalog Number: (103265-234)
Supplier: Southern Biotechnology
Description: FITC Anti-Human Kappa antibody for use in flow cytometry assays.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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The original product is no longer available. The replacement shown is available.
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