You Searched For: brady b403

Catalog Number: (100243-270)

Supplier: Southern Biotechnology

Description: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.

Sale

Catalog Number: (103265-186)

Supplier: Southern Biotechnology

Description: HRP Anti-Human IgG1 Fc antibody for use in ELISA and western blotting assays.

Sale

Catalog Number: (100242-238)

Supplier: Southern Biotechnology

Description: ApoScreen® Annexin V (SB Cat. No. 10040) is a recombinant protein that is expressed in E. coli. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.

Sale

Catalog Number: (100245-458)

Supplier: Southern Biotechnology

Description: The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vβ families, Vβ1 and Vβ2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- subpopulations. The TCR-3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR-3+] cells in the blood and spleen express relatively high levels of the TCR-3/CD3 receptor complex and are “single positive” with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (~ 80% CD4+ vs ~ 20% CD8+).

Sale

Catalog Number: (101442-560)

Supplier: Southern Biotechnology

Description: Purified Rat IgG2b for use as a standard in ELISA.

Sale

Catalog Number: (100245-414)

Supplier: Southern Biotechnology

Description: CD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.

Sale

Catalog Number: (100244-234)

Supplier: Southern Biotechnology

Description: Low Endotoxin/Azide Free Goat F(ab')2 Anti-Human IgM antibody for use in ELISA, flow cytometry, immunocytochemistry, ELISpot, and stimulation assays.

Sale

Catalog Number: (103265-478)

Supplier: Southern Biotechnology

Description: Low Endotoxin/Azide Free Anti-Human IFN-gamma antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, ELISA, ELISpot, immunoprecipitation, multiplex, and neutralization assays.

Sale

Catalog Number: (100245-642)

Supplier: Southern Biotechnology

Description: AP Anti-Human IgM antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.

Sale

Catalog Number: (103260-006)

Supplier: Southern Biotechnology

Description: Goat F(ab')2 Anti-Human Kappa-Alexa Fluor 488 antibody for use in flow cytometry, immunocytochemistry, and western blotting assays.

Sale

Catalog Number: (100243-608)

Supplier: Southern Biotechnology

Description: CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. The monoclonal antibody H1.2F3 augments PMA-induced T-cell proliferation and induces redirected lysis of Fc receptor-bearing target cells by NK cells.

Sale

Catalog Number: (100244-006)

Supplier: Southern Biotechnology

Description: The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. SCF/c-Kit also participates in erythropoiesis in both the bone marrow and spleen. Loss-of-function mutations for SCF/c-Kit lead to a variety of pleiotropic developmental defects while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer. Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas.

Sale

Catalog Number: (101442-688)

Supplier: Southern Biotechnology

Description: Matrix metalloproteinases (MMPs) are a family of at least 23 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-1 has shown substrate specificity to collagens types I, II, III, VII, VIII, X, and XI as well as additional extracellular matrix components including gelatin, fibronectin, laminin, and proteoglycan.

Sale

Catalog Number: (100242-752)

Supplier: Southern Biotechnology

Description: Goat Anti-Mouse IgG3-CY5 antibody with minimal reactivity to human proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Sale

Catalog Number: (103264-552)

Supplier: Southern Biotechnology

Description: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.

Sale

Catalog Number: (103264-922)

Supplier: Southern Biotechnology

Description: SouthernBiotech’s Mouse Immunoglobulin Panel contains 0.1 mg of mouse IgA, mouse IgG1, mouse IgG2a, mouse IgG2b, mouse IgG3, and mouse IgM for use in immunoassays. The kit may be used as positive reference standards for isotyping and standards for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, feces, and ascites.

Sale