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Catalog Number: (100244-916)

Supplier: Southern Biotechnology

Description: Rabbit F(ab')2 Anti-Human IgG(H+L)-HRP antibody for use in ELISA, immunocytochemistry, and western blotting assays.

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Catalog Number: (100245-824)

Supplier: Southern Biotechnology

Description: AP Anti-Human IgG3 Hinge antibody for use in ELISA, immunohistochemistry, western blotting, and ELISpot assays.

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Catalog Number: (103264-776)

Supplier: Southern Biotechnology

Description: Goat Anti-Human IgG Fc-Alexa Fluor 488 antibody for use in western blotting and for other fluorescent assays.

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Catalog Number: (100244-040)

Supplier: Southern Biotechnology

Description: The monoclonal antibody 17-3-3 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype. Reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g, H-2q, and H-2s haplotypes do not express I-E antigen.

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Supplier: Southern Biotechnology

Description: The CD23 antigen is the low affinity IgE Fc receptor, which is a 45-49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells) and can also be found on the surface of T cells, macrophages and platelets. It is distinct from the high affinity IgE receptors found on basophils and mast cells which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.

Catalog Number: (100242-134)

Supplier: Southern Biotechnology

Description: Purified Hamster IgG for use as a control in flow cytometry assays and as a standard in ELISA.

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Catalog Number: (103265-010)

Supplier: Southern Biotechnology

Description: Rabbit Anti-Goat IgG(H+L)-FITC antibody with minimal reactivity to human serum proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

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Catalog Number: (101442-774)

Supplier: Southern Biotechnology

Description: CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. The monoclonal antibody H1.2F3 augments PMA-induced T-cell proliferation and induces redirected lysis of Fc receptor-bearing target cells by NK cells.

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Catalog Number: (100241-952)

Supplier: Southern Biotechnology

Description: Purified Mouse IgG3 for use as a control in flow cytometry, immunocytochemistry, immunoprecipitation, and in vitro assays and as a standard in ELISA.

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Catalog Number: (103265-574)

Supplier: Southern Biotechnology

Description: Goat Anti-Mouse IgG(H+L)-PE antibody with minimal reactivity to human, rat, hamster, goat, sheep, rabbit, chicken, guinea pig, horse, and bovine serum proteins for use in fluorescent assays.

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Catalog Number: (100245-406)

Supplier: Southern Biotechnology

Description: Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells, and 25% of peripheral blood lymphocytes. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

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Catalog Number: (100241-926)

Supplier: Southern Biotechnology

Description: Mouse IgG2a-CY5 for use as a control in flow cytometry and immunohistochemistry / immunocytochemistry assays.

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Catalog Number: (100245-438)

Supplier: Southern Biotechnology

Description: The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike human and mouse in which γδ cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of γδ T cells. The frequency of TCRγδ cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRγδ+ cells in the thymus and blood are CD4-CD8- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRγδ+ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of γδ T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ γδ T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.

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Catalog Number: (100243-232)

Supplier: Southern Biotechnology

Description: CD11b, the integrin αM subunit, combines with CD18, the integrin β2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.

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Catalog Number: (100243-022)

Supplier: Southern Biotechnology

Description: CD106, also known as VCAM-1, is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (e.g., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The M/K-2 monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under non-reducing conditions.

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Catalog Number: (103260-178)

Supplier: Southern Biotechnology

Description: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa ε chain of the CD3/TCR complex.

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