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Catalog Number: (100245-270)

Supplier: Southern Biotechnology

Description: Donkey Anti-Goat IgG(H+L)-Biotin antibody with minimal reactivity to mouse and rat serum proteins for use in ELISA, flow cytometry, immunohistochemistry, western blotting, and multiplex assays.

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Catalog Number: (101442-754)

Supplier: Southern Biotechnology

Description: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 (SB Cat. No. 1600). While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antibody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.

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Catalog Number: (103259-930)

Supplier: Southern Biotechnology

Description: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.

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Catalog Number: (103260-130)

Supplier: Southern Biotechnology

Description: CD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.

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Catalog Number: (100245-476)

Supplier: Southern Biotechnology

Description: Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45low) of thymocytes. The CD45low population contains exclusively thymocytes with the CD3-CD4-CD8low phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8low to CD4+CD8+ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45low subpopulation provides a suitable marker for the CD3-CD4-CD8low immediate precursors to the CD3-CD4+CD8+ thymocytes.

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Catalog Number: (100243-240)

Supplier: Southern Biotechnology

Description: CD11b, the integrin αM subunit, combines with CD18, the integrin β2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.

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Catalog Number: (101442-516)

Supplier: Southern Biotechnology

Description: Mouse IgG2b-APC/CY7 for use as a control in flow cytometry assays.

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Catalog Number: (101442-858)

Supplier: Southern Biotechnology

Description: Biotin Anti-Rabbit IgG antibody for use in ELISA, flow cytometry, immunohistochemistry, and western blotting assays.

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Catalog Number: (103264-740)

Supplier: Southern Biotechnology

Description: Goat Anti-Human IgM-Alexa Fluor 647 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

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Catalog Number: (100242-576)

Supplier: Southern Biotechnology

Description: Goat Anti-Mouse Kappa-TRITC antibody for use in immunohistochemistry / immunocytochemistry assays.

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Catalog Number: (100241-894)

Supplier: Southern Biotechnology

Description: Mouse IgG1-FITC for use as a control in flow cytometry and immunohistochemistry / immunocytochemistry assays.

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Catalog Number: (100242-124)

Supplier: Southern Biotechnology

Description: Rat IgM-PE for use as a control in flow cytometry assays.

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Catalog Number: (100243-000)

Supplier: Southern Biotechnology

Description: The fibrillins are extracellular matrix glycoproteins which occur as a major component of a subset of connective tissue microfibrils. These microfibrils have a beaded appearance and a cross-sectional diameter of 10-12 nm. In elastic tissues, these structures are thought to provide the scaffold onto which elastin is assembled to form elastic fibers, although their function in non-elastic tissues is unclear. There is an aberrant expression of fibrillin in Marfan syndrome. The epitope for the monoclonal antibody 11C1.3 has been found to be within exons 18-20 (a.a. 723-909).

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Catalog Number: (103260-086)

Supplier: Southern Biotechnology

Description: AP Anti-Monkey IgG for use in ELISA and western blotting assays.

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Catalog Number: (100242-838)

Supplier: Southern Biotechnology

Description: Purified Anti-Mouse IgA antibody for use in ELISA, flow cytometry, immunohistochemistry, and western blotting assays.

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Catalog Number: (100243-036)

Supplier: Southern Biotechnology

Description: VLA-4 belongs to the β1-integrin family, which functions both as cell-matrix and cell-cell receptors. As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/Pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. Antibody PS/2 also reacts with human VLA-4.

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