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Catalog Number: (100244-274)

Supplier: Southern Biotechnology

Description: Goat Anti-Human IgG-Biotin antibody for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, immunoprecipitation, ELISpot, and multiplex assays.

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Catalog Number: (100243-912)

Supplier: Southern Biotechnology

Description: CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis, and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 monoclonal antibody M290. 2E7 is reported to have signal-inducing activity in a redirected lysis assay and to costimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro which runs counter to the usual inhibition of CTL by anti-integrins. This costimulatory activity is also shared by the monoclonal antibody M290. The property of costimulation is not unique to the mouse since the combination of anti-human αIEL plus anti-TCR will produce similar proliferation in human cells with the exception that CD8+ cells are not stimulated.

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Catalog Number: (100242-222)

Supplier: Southern Biotechnology

Description: FITC ApoScreen® Annexin V Apoptosis Kit for use in flow cytometry and immunocytochemistry assays.

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Catalog Number: (100243-042)

Supplier: Southern Biotechnology

Description: VLA-4 belongs to the β1-integrin family, which functions both as cell-matrix and cell-cell receptors. As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/Pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. Antibody PS/2 also reacts with human VLA-4.

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Catalog Number: (100243-164)

Supplier: Southern Biotechnology

Description: CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.

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Catalog Number: (103259-826)

Supplier: Southern Biotechnology

Description: Goat Anti-Mouse IgG3-PE/CY5 (SPRD) antibody with minimal reactivity to human proteins for use in flow cytometry assays.

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Catalog Number: (100243-372)

Supplier: Southern Biotechnology

Description: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.

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Catalog Number: (100241-992)

Supplier: Southern Biotechnology

Description: Mouse IgG-PE for use as a control in flow cytometry assays.

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Catalog Number: (100246-046)

Supplier: Southern Biotechnology

Description: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa ε chain of the CD3/TCR complex.

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Catalog Number: (100242-726)

Supplier: Southern Biotechnology

Description: Purified Goat F(ab’)2 Anti-Mouse IgG2b antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, and immunocytochemistry assays.

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Catalog Number: (101190-208)

Supplier: Southern Biotechnology

Description: Mouse IgG-APC/CY5.5 for use as a control in flow cytometry assays.

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Catalog Number: (101442-828)

Supplier: Southern Biotechnology

Description: CY5 Anti-Rat IgG2a antibody for use in flow cytometry and immunohistochemistry assays.

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Catalog Number: (101442-936)

Supplier: Southern Biotechnology

Description: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa ε chain of the CD3/TCR complex.

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Catalog Number: (101442-628)

Supplier: Southern Biotechnology

Description: Low Endotoxin/Azide Free Goat F(ab’)2 Anti-Mouse Kappa antibody for use in ELISA, flow cytometry, immunohistochemistry, ELISpot, and stimulation assays.

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Catalog Number: (100246-264)

Supplier: Southern Biotechnology

Description: CD43, a member of the sialomucin family of cell surface receptors, is a 95-135 kDa type I transmembrane glycoprotein whose variability in molecular weight is dependent upon the nature of the extracellular O-glycans. It is the major sialoglycoprotein on thymocytes, T cells, and neutrophils. It is also present on activated B cells, plasma cells, NK cells, granulocytes, monocytes, macrophages, platelets, and bone marrow hematopoietic stem cells. CD43, which binds to ICAM-1 (CD54), appears to function as an anti-adhesion molecule, inhibiting T cell interactions including T cell killing, and by increasing the threshold for T cell activation.

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Catalog Number: (100242-022)

Supplier: Southern Biotechnology

Description: Goat IgG-PE for use as a control in flow cytometry assays.

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