You Searched For: CUBE BIOTECH

Description: HisPur™ Ni-NTA Spin Plates contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins

Catalog Number: PI88230

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Zinc Reversible Stain Kit provides rapid and highly sensitive detection of protein in polyacrylamide gels immediately after electrophoresis as well as erasability for subsequent transfer or band extraction.

Catalog Number: PI24582

Supplier: Invitrogen

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Description: Imject™ purification buffer salts is a dry powder for preparing 60 mL of solution for buffer-exchange of immunogens following conjugation.

Catalog Number: PI77159

Supplier: Invitrogen

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Description: Plates are optically clear polystyrene with a high-antibody binding 96-well microplate with individual 8-well strips in the plate frame for immunoassay applications.

Catalog Number: PI15031

Supplier: Invitrogen

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Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Catalog Number: 103523-924

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Catalog Number: PI26174

Supplier: Invitrogen

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Description: Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions<i> in vivo</i> using two-hybrid systems.

Catalog Number: PI75768

Supplier: Invitrogen

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Description: LightShift poly(dI-dC) can be used as competitor for nonspecific DNA binding proteins.

Catalog Number: PI20148E

Supplier: Invitrogen

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Description: Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

Catalog Number: PI88433

Supplier: Invitrogen

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Description: SuperBlock™ Blocking Buffer is optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Catalog Number: PI37516

Supplier: Invitrogen

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Description: HRP-Conjugated Streptavidin consists of streptavidin protein that is covalently conjugated to horseradish peroxidase (HRP) enzyme (RZ > 3.0). Streptavidin binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate substrate system. This particular product has been used primarily in sandwich ELISA applications to provide consistent measurement of biotinylated detection antibodies.

Formulation: Provided in a conjugate stabilizer containing 0.1% ProClin Compound as a preservative.
Shipped on cold packs.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is from 1:4,000 to 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Catalog Number: PIN100

Supplier: Invitrogen

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Description: Thermo Scientific Imject Freund's Incomplete Adjuvant is a popular nonspecific stimulator of the immune response for use in antibody production immunization protocols.

Catalog Number: PI77145

Supplier: Invitrogen

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Description: These Immunodiffusion Plates are standard Ouchterlony gel plates containing patterns of six wells around one well for studying and characterizing antibody-antigen binding interactions.

Catalog Number: PI31111

Supplier: Invitrogen

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Description: LightShift® Chemiluminescent EMSA Kit is robust and sensitive system for performing electrophoretic mobility shift assays (EMSAs) to identify and characterize protein:DNA binding interactions.

Catalog Number: PI20148X

Supplier: Invitrogen

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Description: RBS 35 Detergent Concentrate is a general cleaning agent for use on many laboratory surface materials and glassware, including lenses, mirrors, and reflectors.

Catalog Number: PI27852

Supplier: Invitrogen

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Description: These Glutathione spin columns provide a fast, easy-to-use format to purify GST-fusion proteins from cellular lysates.

Catalog Number: PI16104

Supplier: Invitrogen

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