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You Searched For: Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-97065-010

VWR® Bradford Method Protein Assay Kit

Supplier: VWR
Description: The Bradford Protein Assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method. The standard protocol is used for measuring 10-100 μg of protein and the microassay is used for measuring 1-10 μg of protein. For maximum convenience, Bradford Reagent and 0.5 mg/ml BSA standard solution are offered individually or as components of a kit.

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Product Image-10147-342

AzureRed Fluorescent Protein Stain, Azure BioSystems

Catalog Number: (10147-342)
Supplier: Azure Biosystems
Description: AzureRed Fluorescent Protein Stain is a quantitative total protein stain for blots and blots with the sensitivity to detect less than 1 ng of protein per band or spot

SDS Product available on GSA Advantage®


Product Image-89156-498

DPPIV/CD26 Assay Kit for Biological Samples, Enzo Life Sciences

Catalog Number: (89156-498)
Supplier: Enzo Life Sciences
Description: The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered <i>in vivo</i>.
Product Image-89156-048

Annexin V-Cy5 Apoptosis Detection Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: For the detection of apoptotic cells by flow cytometry.

Product Image-89155-974

Caspase-9 Colorimetric Assay Kit, Enzo Life Sciences

Catalog Number: (89155-974)
Supplier: Enzo Life Sciences
Description: Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate LEHD-pNA.
Image Unavailable-89165-248

Auto-Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-248)
Supplier: Enzo Life Sciences
Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation
Image Unavailable-76002-366

ADAM17 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (76002-366)
Supplier: Enzo Life Sciences
Description: Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
Product Image-89156-036

Annexin V-Cy3 Apoptosis Detection Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: For the detection of apoptotic cells by flow cytometry.

Product Image-89165-452

Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-452)
Supplier: Enzo Life Sciences
Description: Versatile tool for generation of ubiquitin-E2 thioesters.
Image Unavailable-10051-878

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Supplier: Tonbo Biosciences
Description: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry

Product Image-10033-580

Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (10033-580)
Supplier: Enzo Life Sciences
Description: The Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit give you all the benefits of chemiluminescent detection without any of the side effects.
Product Image-89141-844

Rap1 activation kit, Enzo Life Sciences

Catalog Number: (89141-844)
Supplier: Enzo Life Sciences
Description: For the measurement of active Rap1 in cell lysates of human, mouse, and rat origin.
Product Image-89141-850

p70 S6K Activity Kit, Enzo Life Sciences

Catalog Number: (89141-850)
Supplier: Enzo Life Sciences
Description: For the measurement of p70 S6K activity in partially purified, purified, or crude enzyme preparations from any species.
Image Unavailable-89165-924

eFluxx-ID® Multidrug Resistance Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP

SDS

Product Image-10048-828

QBT Fatty Acid Uptake Assay Kit, Molecular Devices

Supplier: Molecular Devices
Description: The QBT™ Fatty Acid Uptake Assay is a homogeneous assay amenable to high-throughput screening.

Product Image-10033-576

FLUOR DE LYS® SIRT5 fluorometric drug discovery assay kit, Enzo Life Sciences

Catalog Number: (10033-576)
Supplier: Enzo Life Sciences
Description: The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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