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You Searched For: Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-97064-924

VWR® Bradford Method Protein Assay Kit

Supplier: VWR
Description: The Bradford Protein Assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method. The standard protocol is used for measuring 10-100 μg of protein and the microassay is used for measuring 1-10 μg of protein. For maximum convenience, Bradford Reagent and 0.5 mg/ml BSA standard solution are offered individually or as components of a kit.

SDS Certificates

Product Image-10147-342

AzureRed Fluorescent Protein Stain, Azure BioSystems

Catalog Number: (10147-342)
Supplier: Azure Biosystems
Description: AzureRed Fluorescent Protein Stain is a quantitative total protein stain for blots and blots with the sensitivity to detect less than 1 ng of protein per band or spot

SDS


Image Unavailable-76002-366

ADAM17 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (76002-366)
Supplier: Enzo Life Sciences
Description: Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
Product Image-89165-452

Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-452)
Supplier: Enzo Life Sciences
Description: Versatile tool for generation of ubiquitin-E2 thioesters.
Product Image-89156-036

Annexin V-Cy3 Apoptosis Detection Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: For the detection of apoptotic cells by flow cytometry.

Product Image-95017-766

2-D Quant Kit, Cytiva

Catalog Number: (95017-766)
Supplier: GE Healthcare - Life Sciences
Description: 2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

SDS


Product Image-89155-976

Caspase-9 Fluorometric Assay Kit, Enzo Life Sciences

Catalog Number: (89155-976)
Supplier: Enzo Life Sciences
Description: Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate LEHD-AFC.
Product Image-89155-950

Caspase-1 Colorimetric Assay Kit, Enzo Life Sciences

Catalog Number: (89155-950)
Supplier: Enzo Life Sciences
Description: Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate YVAD-pNA.
Product Image-89155-960

Caspase-3 Fluorometric Assay Kit, Enzo Life Sciences

Catalog Number: (89155-960)
Supplier: Enzo Life Sciences
Description: Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.
Product Image-10033-574

FLUOR DE LYS® Green SIRT5 Fluorometric Drug Discovery Assay Kit, Enzo Life Sciences

Catalog Number: (10033-574)
Supplier: Enzo Life Sciences
Description: FLUOR DE LYS® Green SIRT5 fluorometric drug discovery kit is a sensitive fluorometric assay that measures the lysyl desuccinylase activity of the recombinant human SIRT5.
Product Image-89155-878

Annexin V-FITC Apoptosis Detection Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: For the detection of apoptotic cells by flow cytometry.

Product Image-89141-842

Rac1 activation kit, Enzo Life Sciences

Catalog Number: (89141-842)
Supplier: Enzo Life Sciences
Description: For the measurement of active Rac1 in cell lysates of human, mouse, rat, dog, and chicken origin.
Product Image-89156-550

Calcineurin Phosphatase Assay Kit, Enzo Life Sciences

Catalog Number: (89156-550)
Supplier: Enzo Life Sciences
Description: This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin
Product Image-89165-910

PROTEOSTAT® Protein Aggregation Assay, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution

Product Image-89141-228

SOD Activity Kit, Enzo Life Sciences

Catalog Number: (89141-228)
Supplier: Enzo Life Sciences
Description: ELS offers a large collection of superoxide dismutase products for oxidative stress research.
Product Image-89155-962

Caspase-5 Colorimetric Assay Kit, Enzo Life Sciences

Catalog Number: (89155-962)
Supplier: Enzo Life Sciences
Description: Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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