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You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Product Image-470177-182

Ward's® Plasmid DNA for Transformations

Supplier: NEW ENGLAND BIOLABS INC SE
Description: These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.

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pBlueScript II Vectors, Agilent Technologies

Supplier: Agilent Technologies
Description: The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Certificates

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AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: Agilent Technologies
Description: The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

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Vector, E. coli BL21, Cytiva

Catalog Number: (95017-592)
Supplier: GE Healthcare - Life Sciences
Description: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

SDS


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AAV Helper-Free System, Agilent Technologies

Supplier: Agilent Technologies
Description: The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

Certificates

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Expression vectors, pGEX

Supplier: GE Healthcare - Life Sciences
Description: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

SDS Certificates

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AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: Agilent Technologies
Description: The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.

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pJY2 Plasmid, Enzo Life Sciences

Catalog Number: (89157-406)
Supplier: Enzo Life Sciences
Description: pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
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Polyadenylic acid (poly-A)

Supplier: GE Healthcare - Life Sciences
Description: Polyadenylic acid (poly-A) is a single stranded RNA homopolymer.

SDS

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InterPlay Adenoviral TAP Systems, Agilent Technologies

Supplier: Agilent Technologies
Description: The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

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SureVector, Vectors, Agilent Technologies

Supplier: Agilent Technologies
Description: Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

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Vector, pUC 18, MP Biomedicals

Catalog Number: (IC821226)
Supplier: MP Biomedicals
Description: pUC 18 is a small vector of approximately 2.7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

SDS


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InterPlay Adenoviral TAP System, Agilent Technologies

Supplier: Agilent Technologies
Description: The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

Image Unavailable-470202-882

pBR322 from Escherichia coli, MP Biomedicals

Catalog Number: (470202-882)
Supplier: MP Biomedicals
Description: pBR322 is a 4363 bp vector purified from <B>Escherichia coli</B>.
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Novagen® pET-28a(+) DNA, MilliporeSigma

Catalog Number: (EM69864-3)
Supplier: MilliporeSigma
Description: The pET-28a-c(+) vectors carry an N-terminal His Tag/thrombin/T7 Tag configuration plus an optional C-terminal His Tag sequence.

Certificates


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Novagen® pET-28b(+) DNA, MilliporeSigma

Catalog Number: (EM69865-3)
Supplier: MilliporeSigma
Description: The pET-28a-c(+) vectors carry an N-terminal His Tag®/thrombin/T7 Tag® configuration plus an optional C-terminal His Tag sequence.

Certificates


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