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You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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AEC peroxidase substrate kit, Enzo Life Sciences

Catalog Number: (89165-862)
Supplier: Enzo Life Sciences
Description: The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture.
Image Unavailable-89156-424

BIOMOL® Green, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: BIOMOL Green™ provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL Green™ doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL Green™ is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL Green™ has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL Green™ stops the reaction and begins color development, which is read 20-30 min. later.BIOMOL Green™ may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Product Image-89156-524

FLUOR DE LYS® SIRT2 Fluorometric Drug Discovery Assay Kit, Enzo Life Sciences

Catalog Number: (89156-524)
Supplier: Enzo Life Sciences
Description: A FLUOR DE LYS® fluorescent assay system
Product Image-89156-512

FLUOR DE LYS® HDAC6 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-512)
Supplier: Enzo Life Sciences
Description: A Fluor de Lys® fluorescent assay system
Product Image-89156-520

LSD1 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-520)
Supplier: Enzo Life Sciences
Description: The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.
Product Image-89156-490

Matrix Metalloproteinase-20 (MMP-20) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-490)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89160-360

Cyclic Nucleotide Phosphodiesterase Assay Kit Components, Enzo Life Sciences

Supplier: Enzo Life Sciences

Product Image-89156-474

Matrix Metalloproteinase-13 (MMP-13) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-474)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
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PLAQPRO® Lp-PLA2 Assay, Enzo Life Sciences

Catalog Number: (76013-838)
Supplier: Enzo Life Sciences
Description: Enzyme activity kit to measure Lp-PLA2 activity in  serum samples
Product Image-89156-466

Matrix Metalloproteinase-2 (MMP-2) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-466)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-10033-638

PROTEOSTAT® Thioredoxin-1 Assay Kit, Enzo Life Sciences

Catalog Number: (10033-638)
Supplier: Enzo Life Sciences
Description: This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.
Product Image-10033-748

Matrix Metalloproteinase (MMP) Inhibitor Profiling Kit, Fluorometric RED, Enzo Life Sciences

Catalog Number: (10033-748)
Supplier: Enzo Life Sciences
Description: The MMP inhibitor profiling kit, fluorometric (also known as fluorimetric) RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6’-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties.
The assays are performed in a convenient 96-well microplate format. The compound NNGH is also included as a prototypic control inhibitor.
Product Image-89156-450

Matrix Metalloproteinase-3 (MMP-3) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-450)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89156-470

Matrix Metalloproteinase-9 (MMP-9) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-470)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89156-458

Matrix Metalloproteinase-1 (MMP-1) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-458)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89156-416

MMP Inhibitor Profiling Kit, Colorimetric, Enzo Life Sciences

Catalog Number: (89156-416)
Supplier: Enzo Life Sciences
Description: A QUANTIZYME® Assay System Complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes. The assays are performed in a convenient 96-well microplate format using a chromogenic substrate1,2 detected at 412nm. Included are active MMP enzymes, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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