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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-102715-942

ELECTROLIGASE®, New England Biolabs

Catalog Number: (102715-942)
Supplier: New England Biolabs (NEB)
Description: ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.

Small Business Enterprise


Product Image-PAM8225

LigaFast Rapid DNA Ligation System, Promega

Supplier: Promega Corporation
Description: T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Certificates

Image Unavailable-PAP2301

Bacterial Strain NM522, Promega

Catalog Number: (PAP2301)
Supplier: Promega Corporation
Description: NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection.
Product Image-PAC8820

Flexi System, Transfer, 100 transfer reactions, Promega

Catalog Number: (PAC8820)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Certificates


Product Image-200064-420

9°N™ DNA Ligase, New England Biolabs

Catalog Number: (200064-420)
Supplier: New England Biolabs (NEB)
Description: 9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Small Business Enterprise


Image Unavailable-EM70189-4

pT7Blue Perfectly Blunt® Cloning Kit, Novagen®, EMD Millipore

Supplier: MilliporeSigma
Description: The pT7Blue cloning kit features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.

Certificates

Product Image-102715-946

Instant Sticky-end Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Small Business Enterprise

Product Image-101228-182

T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Certificates Small Business Enterprise

Product Image-102877-808

NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs

Catalog Number: (102877-808)
Supplier: New England Biolabs (NEB)
Description: The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Small Business Enterprise


Product Image-PAC8640

Flexi System, Entry/Transfer, 5 entry and 20 transfer reactions, Promega

Catalog Number: (PAC8640)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Product Image-76407-348

Salt-T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

Product Image-102715-940

Blunt/TA Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

Small Business Enterprise

Product Image-PAM1794

T4 DNA Ligase, Promega

Catalog Number: (PAM1794)
Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning

Certificates


Product Image-101640-956

T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Small Business Enterprise

Product Image-103307-256

RtcB Ligase

Catalog Number: (103307-256)
Supplier: New England Biolabs (NEB)
Description: RtcB Ligase is expressed as a His-tagged fusion in <i>E. coli</i>.

Small Business Enterprise


Product Image-102969-104

NEBNext Ultra II Ligation Module, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Small Business Enterprise

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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