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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-101417-960

T4 RNA Ligase 2 Truncated, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

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Image Unavailable-PAG6050

HaloTag Cloning Starter System, 1 each, Promega

Catalog Number: (PAG6050)
Supplier: Promega Corporation
Description: HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.
Product Image-103307-256

RtcB Ligase

Catalog Number: (103307-256)
Supplier: New England Biolabs (NEB)
Description: RtcB Ligase is expressed as a His-tagged fusion in <i>E. coli</i>.

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Product Image-101386-088

T4 RNA Ligase 2, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

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Product Image-101227-654

QUICK LIGATION™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

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Image Unavailable-PAC1263

T4 DNA Ligase 10X Buffer, Promega

Catalog Number: (PAC1263)
Supplier: Promega Corporation
Description: For ligation of DNA ends.

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Image Unavailable-77676-368

T4 DNA Ligase - High Concentration

Supplier: EMPIRICAL BIOSCIENCE
Description: T4 DNA Ligase - High Concentration

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Product Image-102877-596

T4 RNA Ligase 1 (ssRNA Ligase) (30,000 units/ml), New England Biolabs

Catalog Number: (102877-596)
Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)

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Product Image-102715-906

Thermostable 5' AppDNA/RNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

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Product Image-102715-912

Gibson Assembly® Cloning Kit, New England Biolabs

Catalog Number: (102715-912)
Supplier: New England Biolabs (NEB)
Description: The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.

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Product Image-102500-052

Gibson Assembly Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Gibson Assembly was developed by Dr

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Product Image-76538-670

NEBridge Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Product Image-76717-036

EnGen® Spy Cas9 HF1

Supplier: New England Biolabs (NEB)
Description: EnGen® Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from streptococcus pyogenes with reduced non-specific DNA cleavage.

Image Unavailable-77394-704

Seamless Assembly Master Mix (2X)

Supplier: REVACC INC
Description: This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Product Image-102500-168

T3 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

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Product Image-101228-154

Taq DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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