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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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T7Select®10-3 Cloning Kit, MilliporeSigma

Catalog Number: (EM70550-3)
Supplier: MilliporeSigma
Description: Novagen's T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7.

Certificates


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BioXp® Select DNA Cloning Gibson Assembly Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.

New Product

Product Image-101417-940

QUICK BLUNTING™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Small Business Enterprise

Product Image-102877-564

NEB® PCR Cloning Kit, New England Biolabs

Catalog Number: (102877-564)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

SDS Small Business Enterprise


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NxGen™ T4 DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

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Ampligase® Thermostable DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: Highly specific thermostable DNA ligase for high-temperature applications

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Mutagenesis Kits QuikChange, Agilent Technologies

Supplier: Agilent Technologies
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

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Gigapack III Packaging Extracts, Agilent Technologies

Supplier: Agilent Technologies
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Image Unavailable-EM71229-3

pSTBlue-1 Perfectly Blunt® Giga Cloning Kit, EMD Millipore

Catalog Number: (EM71229-3)
Supplier: MilliporeSigma
Description: The pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency (>1×109 cfu/µg). NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue-white screening capability (with appropriate plasmids) and recA endA mutations, which facilitate high yields of excellent-quality plasmid DNA. Cells are provided in a 50 µL, single-use volume that eliminates the need to dispense, freeze/thaw or waste partially used vials.

Certificates


Product Image-102715-908

T4 RNA Ligase 2 Truncated, KQ, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Small Business Enterprise

Product Image-103218-906

NEBuilder HiFi DNA Assembly Bundle for Large Fragments, New England Biolabs

Catalog Number: (103218-906)
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Small Business Enterprise


Product Image-101228-192

E. coli DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Small Business Enterprise

Image Unavailable-PAP9801

Bacterial Strain JM109(DE3), Glycerol Stock, Promega

Catalog Number: (PAP9801)
Supplier: Promega Corporation
Description: Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Certificates


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T4 DNA Ligase, Promega

Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning.

Certificates

Product Image-101228-190

T4 RNA Ligase 1 (ssRNA Ligase), New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Small Business Enterprise

Product Image-102715-946

Instant Sticky-end Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Small Business Enterprise

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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