Blunt/TA Ligase Master Mix, New England Biolabs

Supplier: W.u.G. Dickgießer GmbH
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M0367L M0367S
102715-938EA 462 USD
102715-938 102715-940
Blunt/TA Ligase Master Mix, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.


This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20°C. Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.

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