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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-99902-306

Pfu DNA Ligase, Agilent Technologies

Catalog Number: (99902-306)
Supplier: Agilent Technologies
Description: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
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BioXp® Select DNA Cloning Golden Gate Assembly BsmBI Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.

New Product

Product Image-76299-644

NEB Cloning Competent E. coli Sampler, New England Biolabs

Catalog Number: (76299-644)
Supplier: New England Biolabs (NEB)
Description: The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.
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BioXp® Select DNA Cloning and Amplification Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.

New Product

Product Image-10820-618

Gibson Assembly® ULTRA Kits and Master Mixes (2X)

Supplier: SGI - DNA Synthetic Genomics
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion.

SDS

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BIOXP® 9600 Select Plasmid AMP

Supplier: SGI - DNA Synthetic Genomics
Description: BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

Product Image-95040-312

T4 DNA Ligase, Cytiva

Catalog Number: (95040-312)
Supplier: GE Healthcare - Life Sciences
Description: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
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BIOXP® Select Plasmid AMP

Supplier: SGI - DNA Synthetic Genomics
Description: BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

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BIOXP® 9600 Select Clone and AMP

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.

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Gibson Assembly® HIFI 1-Step Kits and Master Mixes (2X)

Supplier: SGI - DNA Synthetic Genomics
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion.

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BioXp® Select DNA Cloning Golden Gate Assembly, BsaI Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.

New Product

Product Image-76326-026

NEB® Golden Gate Assembly Kits (BsaI-HF®v2), New England Biolabs

Supplier: New England Biolabs (NEB)
Description: This NEB Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. The pGGA destination plasmid (included), provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable <i>in vitro</i> transcription.

Product Image-102969-096

NEB PCR Cloning Kit (Without Competent Cells), New England Biolabs

Catalog Number: (102969-096)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

Small Business Enterprise


Product Image-ST200550

Random Mutagenesis Kits GeneMorph II, Agilent Technologies

Supplier: Agilent Technologies
Description: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

SDS

Image Unavailable-10801-154

DNA Ligase T4

Supplier: Rockland Immunochemical
Description: DNA Ligase T4 Molecular Biology Grade

Product Image-95040-726

StrataClone PCR Cloning Kits, Agilent Technologies

Supplier: Agilent Technologies
Description: The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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