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Catalog Number: (100243-288)
Supplier: Southern Biotechnology
Description: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.


Catalog Number: (100242-780)
Supplier: Southern Biotechnology
Description: Purified Anti-Mouse IgE antibody for use in ELISA, flow cytometry, immunohistochemistry, western blotting, and ELISpot assays.


Catalog Number: (100245-244)
Supplier: Southern Biotechnology
Description: Sheep Anti-FITC-HRP for use in ELISA and immunohistochemistry assays.


Catalog Number: (103264-626)
Supplier: Southern Biotechnology
Description: The αβ TCR is expressed on T lymphocytes of all mouse strains tested. The H57-597 monoclonal antibody does not react with γδ TCR-bearing cells. Plate-bound or soluble H57-597 activates αβ TCR-bearing T cells. In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis. In vivo administration of H57-597 has been shown to deplete αβ TCR-bearing cells to near completion and prevent graft rejection.


Catalog Number: (103259-932)
Supplier: Southern Biotechnology
Description: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.


Catalog Number: (103264-194)
Supplier: Southern Biotechnology
Description: Rat IgG1-AP for use as a control in immunocytochemistry assays.


Catalog Number: (100244-770)
Supplier: Southern Biotechnology
Description: Goat Anti-Rabbit IgG(H+L)-AP antibody with minimal reactivity to mouse and human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (100243-850)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b. NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).


Catalog Number: (100246-254)
Supplier: Southern Biotechnology
Description: CD42b, also known as GPIbα, is a two-chain membrane glycoprotein that forms a noncovalent complex with CD42a (GPIX) on the surface of platelets and megakaryocytes. Binding of the GPIb/GPIX heterodimer to von Willebrand factor bound to exposed subendothelial surfaces is essential for platelet adhesion at sites of injury.


Catalog Number: (100242-534)
Supplier: Southern Biotechnology
Description: Goat F(ab')2 Anti-Mouse IgG(H+L)-PE/CY5 (SPRD) antibody with minimal reactivity to human proteins for use in flow cytometry assays.


Catalog Number: (100242-082)
Supplier: Southern Biotechnology
Description: Purified Rat IgG2a for use as a control in flow cytometry, immunohistochemistry, immunoprecipitation, and in vitro assays and as a standard in ELISA.


Catalog Number: (100243-430)
Supplier: Southern Biotechnology
Description: The lymphocyte Fcγ receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fcγ type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying FcγR+ cells, monoclonal antibodies to the FcγII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcγR for parasitic invasion. The monoclonal antibody 93 recognizes a conformational epitope formed by FcγII and FcγIII receptors.


Supplier: Southern Biotechnology
Description: Cell Staining Buffer maximizes fluorescent signal intensity generated by pH sensitive fluorochromes.

Catalog Number: (100246-232)
Supplier: Southern Biotechnology
Description: The CD35 antigen is composed of four different allotypes having different molecular weights [A (or F) - 160 kDa, B (or S) - 190 kDa, C - 220 kDa, and D - 250 kDa]. It is the receptor for C3b and is found on B cells, a subset of T cells, monocytes, neutrophils, follicular dendritic cells, erythrocytes, and kidney glomelular podocytes. CD35 mediates neutrophil and monocyte phagocytosis of particles coated with C3b and/or C4b. This interaction also enhances phagocytosis mediated by the Fc receptors.


Catalog Number: (100243-566)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.


Catalog Number: (100244-616)
Supplier: Southern Biotechnology
Description: FITC Anti-Rat IgG2c antibody for use in flow cytometry and immunohistochemistry assays.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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