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Protein production - FectoPRO®

The FectoPRO® Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (<1 μg/ml of cell culture).

  • Amazing protein and antibody production yields
  • Cost-effective Transient Gene Expression using lower plasmid DNA amount
  • Compatible with various mammalian expression media & cells systems
  • Compliant with biomanufacturing guidelines
  • Reproducible production yields

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Description

FectoPRO® enables amazingly high protein and antibody yields in both CHO and HEK-293 cells grown in suspension in serum-free media.

FectoPRO ® gives amazing antibody production yields in suspension CHO cells. Cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyle™ CHO Expression Medium and transfected with FectoPRO ® (0.8 µg DNA/ml) or FreeStyle™ MAX Reagent (1.25 µg DNA/ml) following recommended protocols for the respective reagents. Recombinant mouse IgG production yield was assayed 1 to 6 days after transfection using protein G biosensor (fortéBIO ® octet RED96 system). FreeStyle™ is a trademark of Life Technologies™ Corporation. Data kindly provided by ProteoGenix SAS (France).

The duration of protein production processes has increased in the past few years, and most of the protein production processes now lasts 1-2 weeks to obtain optimal amount of produced protein. FectoPRO® has been developed to ensure sustained protein production over several days, while maintaining high cell viability for longer lasting protein production.

FectoPRO ® gives sustained protein production in suspension cells. FreeStyle™ HEK-293F cells were seeded at 1 x 106cells/ml in 30 ml of FreeStyle™ 293 Expression Medium and transfected with FectoPRO ® + FectoPRO ® Booster (0.5 µg DNA/ml) following the standard protocol. IgG3-Fc production was assayed after different timepoints by protein G affinity quantification (HPLC). Similar results are obtained with FreeStyle™ CHO-S (data not shown).

Unlike other commercially available reagents, FectoPRO® transfection kit is suitable for both CHO and HEK-293 cells. The reagent is compatible with various media formulations; hence lab implementation is easy and straightforward.

Protein yields using FectoPRO ® in different culture media for CHO cells.
Protein yields using FectoPRO ® in different culture media for HEK-293 cells.

FectoPRO® is compliant with biomanufacturing guidelines. Indeed, FectoPRO® is guaranteed free of animal-origin components, and is chemically defined. Systematic lot management and release testing is performed for each lot produced. Furthermore FectoPRO® transfection kit undergoes advanced quality controls: protein productivity, cell viability, and complete sterility.

Examples of citations

Batonick, M., Kiss, M.M., Fuller, E.P., Magadan, C.M., Holland, E.G., Zhao, Q., Wang, D., Kay, B.K., Weiner, M.P. (2016). pMINERVA: A donor-acceptor system for the in vivo recombineering of scFv into IgG molecules, J Immunol Methods, doi: 10.1016/j.jim.2016.02.003.

Ferreira de Freitas, R., Eram, M. S., Smil, D., Szewczyk, M. M., Kennedy, S., Brown, P. J., Santhakumar, V., Barsyte-Lovejoy, D., Arrowsmith, C. H., Vedadi, M., Schapira, M. (2016). Discovery of a Potent and Selective Coactivator Associated Arginine Methyltransferase 1 (CARM1) Inhibitor by Virtual Screening, J Med Chem, doi: 10.1021/acs.jmedchem.6b00668.

Li, M., Yang, S., Xu, D. (2016). Heparan Sulfate Regulates the Structure and Function of Osteoprotegerin in Osteoclastogenesis, J Biol Chem, doi: 10.1074/jbc.M116.751974.

Testimonials

“I have to admit I had low expectations for FectoPRO®. Perhaps a modest improvement in transfection … an extra 5-10%. J. did her first experiment yesterday and analyzed it today and the result was remarkable. I’m shocked by both the efficiency of transfection and the brightness of the GFP.”
Thomas C., Biotech, Cambridge, MA, United States

“2 x the yield for half the amount of transfection reagent = 4 x less cost. It is also very easy to use.”
Jean-Philippe J., Hospital for Sick Children, Canada"

Product selection

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PEIpro is a transfection reagent developped for large scale virus production either in adherent cells grown in presence of serum or in suspension HEK-293 and CHO cells grown in low serum or serum-free media.