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Supplier: EMPIRICAL BIOSCIENCE
Description: The qPCR MasterMix is supplied as a 2X product and is a ready to use HotStart qPCR MasterMix with EvaGreen® dye, FlashTaq DNA polymerase, dCTP, dGTP, dATP, dTTP, and MgCl2. EvaGreen® is a dsDNA-binding dye. EvaGreen® possesses similar absorption and emission spectra as SYBR® Green I.

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Supplier: EMPIRICAL BIOSCIENCE
Description: This enzyme is a thermolabile uracil N-glycosylase (UNG) obtained from Atlantic cod (Gadus morhua). UNG enzymes specifically hydrolyze the N-glycosidic bond of uracil residues in ss/dsDNA molecules leaving an abasic site in the strand but will not react with RNA.

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Supplier: EMPIRICAL BIOSCIENCE
Description: 2X InhibiTaq™ Multiplex PLUS HotStart MasterMix.

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Supplier: EMPIRICAL BIOSCIENCE
Description: Proteinase K is a broad-spectrum endopeptidase derived from the fungus Tritirachium album. The native enzyme has a size of 26 to 28 kDa and preferentially cleaves peptide bonds adjacent to aliphatic and aromatic amino acid residues of both native and denatured proteins.

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Supplier: EMPIRICAL BIOSCIENCE
Description: Cas9 is an RNA-guided endonuclease that is naturally expressed by <i>Streptococcus pyogenes</i>. Catalytically active ribonucleoprotein (RNP) complexes are produced by incubating Cas9 with a sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), which together activate the endonuclease and guide it to the ~20-nucleotide protospacer sequence in target dsDNA. Alternatively, Cas9 can be complexed with a synthetic guide RNA (sgRNA) that encodes both the crRNA and tracrRNA in a single RNA strand.

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Supplier: EMPIRICAL BIOSCIENCE
Description: Reverse transcriptase enzymes are RNA-directed DNA polymerases that catalyzes first strand cDNA synthesis from oligonucleotide primed RNA templates.

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Supplier: EMPIRICAL BIOSCIENCE
Description: FlashTaq™ HotStart 2X MasterMix contains FlashTaq HotStart DNA Polymerase, a chemically modified HotStart Taq DNA polymerase.

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Supplier: EMPIRICAL BIOSCIENCE
Description: 2X InhibiTaq™ hot start qPCR master mix is a 2X ready-to-use master mix for quantitative real time evaluation of DNA using dye- or fluorescent probe-based detection. InhibiTaq™ hot start qPCR master mix contains a chemically modified taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95 °C.

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Supplier: EMPIRICAL BIOSCIENCE
Description: MasterMix supplied as a 2X reaction buffer with Loading Dye, dCTP, dGTP, dATP, dTTP, MgCl2 and Taq DNA polymerase.

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Supplier: EMPIRICAL BIOSCIENCE
Description: T4 DNA Ligase - High Concentration

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Supplier: EMPIRICAL BIOSCIENCE
Description: Phu-50 High Fidelity Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5 - 3' direction but does not possess a 5 - 3' exonuclease replacement activity. Its inherent 3 - 5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.

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Supplier: EMPIRICAL BIOSCIENCE
Description: Contains blue and yellow loading dyes and a density agent that allow reactions to be loaded directly onto agarose gels and make monitoring progress during electrophoresis easy.

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Supplier: EMPIRICAL BIOSCIENCE
Description: Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FASTaq™ is completely suppressed prior to activation, and regains full enzymatic activity after 10 min at 95 °C. Taq DNA Polymerase gene is isolated from <i>Thermus aquaticus</i> YT1 and expressed in <i>E. coli</i>.

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Supplier: EMPIRICAL BIOSCIENCE
Description: Empirical's ActiTemp-50 RTScript™ Reverse Transcriptase is a modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. The enzyme is Warm-Start modified with a proprietary RNA Aptamer.

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Supplier: EMPIRICAL BIOSCIENCE
Description: BST Prime is a recombinantly produced <i>Bacillus stearothermophilus</i> DNA polymerase I, large fragment (BST DNA Polymerase, large fragment).

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Catalog Number: (77676-346)
Supplier: EMPIRICAL BIOSCIENCE
Description: ROX Reference Dye

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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* Please note if your account is within the State of California two of these pieces of identification will be required.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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