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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Extralink-Lite PEGDA is a thiol-reactive crosslinker designed for use in HyStem kits.

Catalog Number: (103792-942)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Gelin-S is a thiol-modified gelatin that comes as a lyophilized powder. Gelin-S is a component of the HyStem®-C and HyStem®-HP kits, but can be purchased separately here.

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Catalog Number: (103700-642)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.

VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is sterile filtered and is supplied as a ready to use solution.

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Catalog Number: (103792-922)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: CHP can specifically bind to denatured collagen.

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Catalog Number: (103701-728)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Bovine Collagen Type II is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body’s total protein). Over two dozen types of collagen have been described; Type II is found predominantly in hyaline cartilage (50% of the protein in hyaline is collagen type II) and is also found in the vitreous humor of the eye.

The product is supplied as a sterile solution at approximately 0.5 mg per vial at approximately ~.50 mg/ml in 500 mM acetic acid. A certificate of Analysis is available with the purchase of each product. Type II collagen is provided in user-friendly packaging for use and storage. Type II collagen may be used to culture a variety of cell types. Type II collagen is typically used as a coating material for cell culture studies, for the formation of 3D collagen gels, or as an antigen for antibody production.

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Catalog Number: (103701-214)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: HCl is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: HyStem®-HP Hydrogel Kit - The growth factor delivery matrix

Catalog Number: (103792-884)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: TeloCol®-10 is 20 mL of 10 mg/ml, type I bovine telocollagen solution for 2D coatings or 3D hydrogels. TeloCol®-10 has a high viscosity and concentration, and can form firm hydrogels.

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Catalog Number: (103701-196)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds. Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs). Fibronectin is ideal for coating of surfaces and is typically used at a concentration of 1-5 ug/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.

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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Catalog Number: (103701-192)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digest Poly-L-Lysine. For those cell types, Poly-D-Lysine 000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: PureCol® -S collagen is provided as a standard for use in assays where ultra-pure collagen is required. PureCol®-S collagen standard is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. This product is supplied at approximately a 3 mg/ml concentration. The concentration is confirmed by Biuret protein determination assay. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol®-S is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

PureCol® -S is ideal for using as a collagen standard in controlled testing and assay procedures. PureCol®-S is sterile filtered and is supplied as a ready to use solution.

Catalog Number: (103701-176)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: PhotoHA®-IRG is a methacrylated hyaluronic acid (HAMA) kit. These 3D hyaluronic acid hydrogels can be prepared at various concentrations and photocrosslinked (365nm) to provide various gel stiffness. Each kit contains 100 mg of methacrylated hyaluronic acid and irgacure photoinitiator.

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Catalog Number: (103700-636)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Type III Collagen provides structure and strength to connective tissue, is found many places in the body, especially skin, lung, intestinal walls and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. The Type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen’s tissue strengthening function.

This Type III Collagen product is isolated from human placenta and is purified using a multi-step process with approximately 85% Type III collagen with the remainder being comprised of Type I collagen. The product is supplied as a non-sterile powder. A Certificate of Analysis is available with the purchase of each product. Type III collagen product is provided in a user-friendly packaging for use and storage.

Type III Collagen is typically used as a thin coating on tissue culture surfaces or as a control. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type III collagen may be used to culture a variety of cell types.

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Catalog Number: (103792-918)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Methacrylated Hyaluronic Acid with LAP Kit

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Catalog Number: (103700-630)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Nutragen® collagen is known as the standard of all collagens for purity (>99% collagen content), functionality, and the most native-like collagen available. Nutragen® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

Nutragen® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.

Nutragen® collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. Nutragen® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

Nutragen® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Nutragen® collagen is provided in a 50 mL volume and is contained in user-friendly packaging for use and storage. Nutragen® is sterile filtered and is supplied as a ready to use solution.

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