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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: HyStem®-HP Hydrogel Kit - The growth factor delivery matrix

Catalog Number: (103701-728)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Bovine Collagen Type II is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body’s total protein). Over two dozen types of collagen have been described; Type II is found predominantly in hyaline cartilage (50% of the protein in hyaline is collagen type II) and is also found in the vitreous humor of the eye.

The product is supplied as a sterile solution at approximately 0.5 mg per vial at approximately ~.50 mg/ml in 500 mM acetic acid. A certificate of Analysis is available with the purchase of each product. Type II collagen is provided in user-friendly packaging for use and storage. Type II collagen may be used to culture a variety of cell types. Type II collagen is typically used as a coating material for cell culture studies, for the formation of 3D collagen gels, or as an antigen for antibody production.

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Catalog Number: (103792-884)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: TeloCol®-10 is 20 mL of 10 mg/ml, type I bovine telocollagen solution for 2D coatings or 3D hydrogels. TeloCol®-10 has a high viscosity and concentration, and can form firm hydrogels.

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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Catalog Number: (103792-922)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: CHP can specifically bind to denatured collagen.

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Catalog Number: (103701-192)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digest Poly-L-Lysine. For those cell types, Poly-D-Lysine 000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

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Catalog Number: (103701-214)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: HCl is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

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Catalog Number: (103701-196)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds. Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs). Fibronectin is ideal for coating of surfaces and is typically used at a concentration of 1-5 ug/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.

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Catalog Number: (103701-198)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.

Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.

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Catalog Number: (103701-490)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: CytoSoft® Imaging products provide a tool to culture cells on PDMS substrates with various rigidity including 0.2, 0.5, 2, 8, 16, 32, and 64 kPa. This CytoSoft® Imaging plate comes with 1 glass-bottom 96-well plate for high resolution imaging of cells on the selected stiffness.

Catalog Number: (103701-730)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.

The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the e-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Approximately 40% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml.The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

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Catalog Number: (103700-644)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Type IV Collagen is the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha 1-like and one Alpha 2-like chains.

This Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a sterile, lyophilized powder containing 5 mg of Type IV collagen per vial. A Certificate of Analysis is available with the purchase of each product.

Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.

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Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Catalog Number: (103701-224)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in your research.

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Catalog Number: (103701-194)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.
The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. Poly-D-Lysine is supplied in a sterile 5 mg package size.

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