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Catalog Number: (75842-584)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The DATK32 monoclonal antibody specifically reacts with an epitoPEof the Mouseα4β7 integrin (LPAM-1). The two subunits of the LPAM-1 heterodimer are the 154 kDa α4 and the 130 kDa β7. Most mature lymphocytes, a subset of bone marrow cells, and some of the thymic cells express the α4β7 integrin heterodimer. α4β7 enhances the transendothelial migration of lymphocytes, and interacts with several ligands (fibronectin, CD106, and MAdCAM-1). The interaction of DATK32 with the α4β7 induces the α4β7-dependent lymphocyte aggregation and inhibits LPAM-1-dediated cell adhesion.

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Catalog Number: (75842-870)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The MWReg30 monoclonal antibody specifically reacts with mouse CD41, a transmembrane glycoprotein also known as integrin alpha 2b GPIIb. It is expressed on hematopoietic progenitors, megakaryocytes, and platelets. It forms a receptor with CD61 (integrin beta 3) that binds adhesion molecules such as fibrinogen, fibronectin, von Willebrand factor, and thrombin. Defects or absence of CD41 has been found to lead to coagulation disorders. The expression profile of CD150+,CD48-, and CD41- can be used to identify hematopoietic stem cells.

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Catalog Number: (75844-072)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The 10.1 monoclonal antibody specifically reacts with human CD64, a 75kDA type I transmembrane glycoprotein that is the high affinity receptor for IgG. CD64 is expressed on macrophages and dendritic cells and is involved in antibody-dependent cellular toxicity, cytokine release phagocytosis, and endocytosis. The 10.1 antibody is reported to recognize the EC3 epitope of CD64.

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Catalog Number: (75841-560)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The M1/70 monoclonal antibody specifically reacts with the 170 kDa αM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.

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Catalog Number: (75843-274)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily. The PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-H2) are members of the B7 family. Pd-1 contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) and influences the peripheral tolerances and autoimmune diseases in mice. PD-1 is transiently expressed on CD4/CD8 thymocytes, it is upregulated in apoptotic cells, and it is expressed by activated myeloid and T and B cells. The binding of PD-1 to its ligands is blocked by the J43 antibody, which also enhances contact hypersensitivity and exacerbates acute Graft-versus-host disease, Experimental autoimmune encephalomyelitis and NOD diabetes. PD-1 seems to downregulate the immune response, as the development of splenomegaly and breakdown of peripheral tolerance in PD-1 deficient mice suggests.

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Catalog Number: (75844-018)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The RPA-T4 monoclonal antibody specifically binds to the CD4 receptor for the human immunodeficiency virus (HIV). CD4 is a 59 kDa single-chain transmembrane glycoprotein that expressed on the surface of most of the thymocytes, T-helper cells, and in low levels on monocytes and macrophages. CD4 is a co-receptor in the antigen-induced T cell activation, together with the MHC class II. The RPA-T4 antibody is capable of blocking HIV binding and inhibiting syncytium formation, by binding to the D1 domain of the CD4 antigen. The OKT4 and the RPA-T4 monoclonal antibodies recognize different epitopes of CD4 and they do not exhibit cross-block binding.

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Catalog Number: (75841-988)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The A20 monoclonal antibody reacts with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A). It does not cross-react with cells that express CD45.2. The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The A20 antibody inhibits some of the B lymphocytes responses, from CD45.1-expressing mice, to lipopolysaccharides and antigens. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

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Catalog Number: (75844-034)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.

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Catalog Number: (75844-120)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.

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Catalog Number: (75843-782)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The 30-H12 monoclonal antibody specifically binds to MouseCD90.2, an alloantigen known as Thy-1.2, expressed on thymocytes, mature T cells, epithelial cells, neurons, hematopoietic stem cells, and fibroblasts. CD90 is a membrane glycoprotein that regulates the adhesion and signal transduction in T lymphocytes, and the adhesion of thymocytes to thymic stroma. The interaction between 30-H12 and the antibody to the CD3/TCR complex upregulates thymocytes signal transduction and apoptosis and downregulates mature T cell proliferation. The 30-H12 antibody seems to be unable to cross-link with CD90.1.

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Catalog Number: (75843-642)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The 1A8 monoclonal antibody specifically reacts with the 21-25 kDa glycophosphatidylinositol-anchored protein known as Ly-6G, expressed by the granulocytes from the bone marrow and periphery neutrophils. Ly-6G and Ly-6C form the Granulocyte Receptor-1 antigen (GR-1). The binding of the 1A8 antibody to the Ly-6G can be blocked by another antibody, RB6-8C5, which also recognizes Ly-6C. While 1A8 is specific for only Ly-6G, RB6-8C5 also binds to Ly-6C.

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Catalog Number: (75843-172)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The 3D12 monoclonal antibody specifically reacts with human CD197 (CCR7 or EBI-1), a seven-transmembrane G-protein-coupled. It is the receptor for the secondary lymphoid-tissue chemokine (SLC or 6CKine), CCL19, and CCL21. CD197 is expressed on subsets of B and T cells, and mature dendritic cells. The monoclonal 3D12 antibody recognizes the N-terminus of the receptor.

We recommend that this antibody be incubated for at least 45 minutes at 4°C for optimal staining.

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Catalog Number: (75843-474)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The XMG1.2 is a neutralizing antibody that binds with the mouse Interferon-gamma (IFN-γ) protein, a 15 -17 kDa cytokine with significant antibacterial, antiviral, and antitumoral properties. When secreted by natural killer cells and by natural killer T lymphocytes, it regulates the immune response and supports adaptive immunity when produced by Th1 or CD8+ T lymphocytes. IFN-γ plays an important role in the activation, the growth, and the differentiation of the macrophages, B and T lymphocytes, and natural killer cells. It interacts synergically with other cytokines, such as TNF-α, to inhibit proliferation of normal and transformed cells. IFN- γ is the primary cytokine that defines Th-1 cells.The biological activity of IFN-γ is not affected by glycosylation.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

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Catalog Number: (75842-430)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The 2A3 antibody was obtained by hybridization of MouseNS-1 myeloma cells with spleen cells harvested from BALB/c mice immunized with phytohemagglutinin-activated human T cells. The 2A3 antibody is a CD25 clone and used as an Isotype Control for rat IgG2a antibodies.

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Catalog Number: (75843-022)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The PO3.1 monoclonal antibody specifically reacts with CD86, also known as B7-2, a surface 80 kDa receptor and member of the B7 family. CD86 is a costimulatory molecule, expressed on B and T cells, dendritic cells, astrocytes, macrophages, Langerhans cells, and at a low level in newly explanted B and T lymphocytes. CD86 expression on B lymphocytes is upregulated by B-Cell Receptor complex, CD40 and certain cytokine receptors. CD80 (B7-1) and CD 86 (B7-2) are receptors for CD28 and CTLA-4, on the surface of T lymphocytes, enhancing the interactions between B and T cells.

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Catalog Number: (75843-648)

Supplier: BIOGEMS INTERNATIONAL INC.

Description: The JES6-5H4 antibody specifically binds to mouse IL-2, a 17 kDa immunoregulatory cytokine and growth factor for T and B lymphocytes, NK cells, monocytes, and macrophages. IL-2 induces the cytotoxic operations of NK cells, activates B cells, and plays a role in the differentiation of T cells. JES6-5H4 seems to have a neutralizing effect on interleukin-2.

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