Description: Thermo Scientific Pierce BMB is a mid-length, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

Catalog Number: PI22331

Supplier: Invitrogen

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Description: Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Catalog Number: PI20593

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Triethylamine (TEA) is a high-purity solvent that is used in certain HPLC protocols, such as weak anion exchange, to resolve certain tryptic peptides on a reverse-phase column.

Catalog Number: PI25108

Supplier: Invitrogen

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Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Catalog Number: PI22585

Supplier: Invitrogen

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Description: The Thermo Scientific Pierce HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Catalog Number: PI88328

Supplier: Invitrogen

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Description: The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.

Catalog Number: PI89842

Supplier: Invitrogen

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Description: The iodoTMTzero label reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation.

Catalog Number: PI90100

Supplier: Invitrogen

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Description: The Pierce™ Coomassie Plus™ Protein Assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Catalog Number: PI23236

Supplier: Invitrogen

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Description: The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples.

Catalog Number: PI89801

Supplier: Invitrogen

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Description: Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0.5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

Catalog Number: PI77720

Supplier: Invitrogen

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Description: Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

Catalog Number: PI22308

Supplier: Invitrogen

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Description: Pierce™ Recombinant Protein A agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilized for use in IgG purification applications where the native ligand has undesirable effects.

Catalog Number: PI20366

Supplier: Invitrogen

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Description: The Pierce™ His Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.

Catalog Number: PI21277

Supplier: Invitrogen

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Description: The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

Catalog Number: PI20158

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Streptavidin Magnetic Beads accelerate throughput for automated magnetic purification of biotinylated molecules.

Catalog Number: PI88816

Supplier: Invitrogen

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Description: Thermo Scientific™ Pierce™ Protein A/G Magnetic Agarose Beads provide a fast, convenient method for purification of immunoglobulins from serum, cell culture supernatant, or ascites.

Catalog Number: PI78609

Supplier: Invitrogen

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