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Catalog Number: (PI21578)
Supplier: Invitrogen
Description: Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Catalog Number: (PI21629)
Supplier: Invitrogen
Description: Use to develop in-house non-radioactive assay systems.


Catalog Number: (PI33043)
Supplier: Invitrogen
Description: Thermo Scientific Pierce TMEA is a trifunctional crosslinker containing three reactive maleimide groups to enable three-way conjugation and polymerization of sulfhydryl-containing molecules.

Catalog Number: (PI34035)
Supplier: Invitrogen
Description: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt

Catalog Number: (PI34002)
Supplier: Invitrogen
Description: The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.

Catalog Number: (PI22297)
Supplier: Invitrogen
Description: Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Supplier: Invitrogen
Description: Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
Catalog Number: (PI35000)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.

Catalog Number: (PI34065)
Supplier: Invitrogen
Description: Thermo Scientific Pierce™ Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate.

Supplier: Invitrogen
Description: SuperSignal™ ELISA Pico chemiluminescent substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer based assays.
Catalog Number: (PI53104)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA), HPLC Grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Supplier: Invitrogen
Description: Thermo Scientific Pierce MS(PEG)<sub>n</sub> reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)<sub>12</sub> is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
Catalog Number: (PIN600)
Supplier: Invitrogen
Description: Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA procedure. Addition of sulfuric acid stop solution changes the color to yellow (absorbance maximum at 450nm), stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

Catalog Number: (PI22363)
Supplier: Invitrogen
Description: Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).

Catalog Number: (PI22339)
Supplier: Invitrogen
Description: Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.


Catalog Number: (PI22327)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
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  • City or County issued Business License
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  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
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-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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