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Supplier: Invitrogen

Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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Catalog Number: (PI22327)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.

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Catalog Number: (PI88430)

Supplier: Invitrogen

Description: Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce High Sensitivity Streptavidin-HRP is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Supplier: Invitrogen

Description: This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods.

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Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins.

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Catalog Number: (PI90409)

Supplier: Invitrogen

Description: The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MSSensitive—procedure successfully enriches low abundance proteins (low ng range)Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteinsRobust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundance proteins (ng range) with at least 2 peptides identified per proteinThe Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone

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Catalog Number: (PI23013)

Supplier: Invitrogen

Description: Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.

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Catalog Number: (PI20307)

Supplier: Invitrogen

Description: Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids.

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Catalog Number: (PI53104)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA), HPLC Grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

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Catalog Number: (PI90056)

Supplier: Invitrogen

Description: Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

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Catalog Number: (PI45206)

Supplier: Invitrogen

Description: The Pierce™ Melon™ Gel IgG Spin Purification Kit is a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

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Catalog Number: (PI88279)

Supplier: Invitrogen

Description: The Pierce™ Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

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Supplier: Invitrogen

Description: The Thermo Scientific Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

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Catalog Number: (PI26181)

Supplier: Invitrogen

Description: 26181 is supplied as 2 ml of antibody on crosslinked 4% beaded agarose supplied as a 50% slurry in 0. 1M phosphate, 0. 15M NaCl, pH 7. 2 with 0. 05% sodium azide. Specific for the HA peptide YPYDVPDYA. The anti-HA agarose has been used successfully in immunoprecipitation applications. The anti-HA agarose has 3. 5mg mouse anti-HA IgG1 monoclonal antibody conjugated per mL of settled agarose resin. HA Synthetic Peptide is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin. An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.

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Catalog Number: (PI77120)

Supplier: Invitrogen

Description: Imject™ Ovalbumin is a purified ovalbumin (OVA) protein conveniently formulated for preparation of hapten-carrier conjugates.

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