Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit, Thermo Scientific

Supplier: Paul Schöndorf Metallwaren GmbH
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PI16181EA 187.2 USD
PI16181 PI16182
Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit, Thermo Scientific
Assays Enzyme Assays

The Thermo Scientific Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.


  • Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
  • Sensitive—measure bright blue Gaussia and red firefly luciferase activities in the same sample
  • Fast—no quenching step required, unlike in traditional sequential dual assays
  • Multiplex—capable of quantitating two cellular activities in the same sample(s)


Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalisation control. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.


Delivery information: Kit includes Gaussia-Firefly dual assay buffer (5 ml), 1X Coelenterazine (5 µl), D-Luciferin, Lyophilised (3 mg) and 2X Cell Lysis Buffer (6 ml).


Caution: For research use only. Not for use in diagnostic procedures.

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