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Description: Thermo Scientific Halt Protease Inhibitor Cocktail (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction from animal and plant tissues, cultured cells,
yeast or bacteria.

Catalog Number: PI87786

Supplier: Invitrogen

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Description: The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

Catalog Number: PI26157

Supplier: Invitrogen

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Description: Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 ml) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive. Able to tolerate centrifuge speeds up to 20000.

Catalog Number: PI88380

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human <i>in vitro</i> expression systems and bacterial and mammalian cell lysates.

Catalog Number: PI20169

Supplier: Invitrogen

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Description: Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.

Catalog Number: PI26180

Supplier: Invitrogen

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Description: The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.

Catalog Number: PI34002

Supplier: Invitrogen

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Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Catalog Number: PI21935

Supplier: Invitrogen

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Description: Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Catalog Number: PI22297

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.

Catalog Number: PI35000

Supplier: Invitrogen

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Description: SuperSignal™ ELISA Pico chemiluminescent substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer based assays.

Catalog Number: PI37070

Supplier: Invitrogen

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Description: Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Catalog Number: PI37621

Supplier: Invitrogen

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Description: Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA), HPLC Grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Catalog Number: PI53104

Supplier: Invitrogen

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Description: Thermo Scientific Pierce MS(PEG)_n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)₁₂ is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

Catalog Number: PI22687

Supplier: Invitrogen

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Description: Thermo Scientific NeutrAvidin coated plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Catalog Number: PI-15509

Supplier: Invitrogen

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Description: Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA procedure. Addition of sulfuric acid stop solution changes the color to yellow (absorbance maximum at 450nm), stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

Catalog Number: PIN600

Supplier: Invitrogen

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Description: Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).

Catalog Number: PI22363

Supplier: Invitrogen

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