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Catalog Number: (PI26100)

Supplier: Invitrogen

Description: N-Succinimidyl 3-(Acetylthio)propionate (SATP), Pierce™

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Catalog Number: (PI20700)

Supplier: Invitrogen

Description: Thermo Scientific Pierce DMS is dimethyl suberimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of an 8-atom spacer arm.

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Catalog Number: (PI20308)

Supplier: Invitrogen

Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0.1 to 10 mL) protein samples.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

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Supplier: Invitrogen

Description: Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Catalog Number: (PI22305)

Supplier: Invitrogen

Description: Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

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Supplier: Invitrogen

Description: This luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate for Western blotting provides very stable light output especially for documentation with CCD camera imagers with sensitivity in the mid-femto range.

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Catalog Number: (PI22298)

Supplier: Invitrogen

Description: Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).

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Supplier: Invitrogen

Description: The Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

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Supplier: Invitrogen

Description: Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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Supplier: Invitrogen

Description: TMT10plex™ Isobaric Mass Tag Labeling Reagents Sets enable multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).

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Supplier: Invitrogen

Description: Pierce™ C18 Tips are ready-to-use pipette-tip columns of C18 resin that enable fast and efficient capture, concentration, desalting and elution of peptides for MALDI mass spectrometry and other methods.

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Catalog Number: (PI21217)

Supplier: Invitrogen

Description: Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

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Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ Protein A/G Magnetic Agarose Beads provide a fast, convenient method for purification of immunoglobulins from serum, cell culture supernatant, or ascites.

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Catalog Number: (PI22331)

Supplier: Invitrogen

Description: Thermo Scientific Pierce BMB is a mid-length, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

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