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Supplier: MP Biomedicals

Description: This concentrated solution was designed to safely clean laboratory plastics and glassware simply by soaking overnight

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Catalog Number: (IC097740005)

Supplier: MP Biomedicals

Description: Microplate sealing tape used to provide a tight seal on 96-well plates to minimize evaporation and condensation for short and long term storage.

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Supplier: MP Biomedicals

Description: Sodium Selenite, Purity: approx 98%, Cas Number: 10102-18-8, Formula: Na2Seo3, Molecular Weight: 172.94, Appearance: Pale Gray Powder, Synonyms Selenious Acid, Disodium, Disodium Selenite, Natriumselenit, Selenious Acid, Disodium Salt, Cell Culture Reagent, Size: 10 G

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Supplier: MP Biomedicals

Description: Lysing matrix D is for rapid and reliable biological sample lysis from a wide variety of starting materials.

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Catalog Number: (IC7640105)

Supplier: MP Biomedicals

Description: This microplate sealing tape is suitable for all microplates for both short- and long-term storage, providing a tight seal to minimize evaporation and condensation.

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Supplier: MP Biomedicals

Description: Cholic acid is a biochemical solubilizing agent (a non-ionic, non-denaturing detergent). Occurs inconjugation with glycine or taurine in bile of most vertebrates.

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Supplier: MP Biomedicals

Description: Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.

Supplier: MP Biomedicals

Description: Cellobiose is a disaccharide composed of two glucose molecules. These are β-1,4-glycosidically linked. It is reducing sugar, which is readily soluble in polar solvents such as water and ethanol. D-Cellobiose is a substrate of β-glucosidase.

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Supplier: MP Biomedicals

Description: A carbohydrate found in plants and plant exudates.

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Supplier: MP Biomedicals

Description: Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon. According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA. They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones. Billing and Bonner suggest that DNase attacks the histone-free strand of chromatin DNA. Schmidt, et. al.indicate that hydrolysis of the histone-free region of DNA strands accounts for the initial rapid action of the enzyme on chromatin. Bollum reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin.

Supplier: MP Biomedicals

Description: Chloride form quaternary ammonium functionality.Strongly basic, macroreticular resin of moderately high porosity with benzyltrialkylammonium functionality.

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Supplier: MP Biomedicals

Description: L-α-Glycerophosphoryl Choline, Cadmium Chloride Complex (from egg yolk) ∼98%, white powder

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Catalog Number: (IC096040805)

Supplier: MP Biomedicals

Description: Uncoated areas have a total wettable surface so that liquid spreads evenly over the area of each well. For cell attachment and growth, the slides require only a simple washing in deionized water or detergent followed by sterilization.

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Supplier: MP Biomedicals

Description: Mannitol is carbohydrate found in plants and plant exudates.

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Supplier: MP Biomedicals

Description: Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.

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Supplier: MP Biomedicals

Description: Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

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