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Supplier: Southern Biotechnology
Description: The CD45 antigen is an essential regulator of leukocyte activation and development and is now known to be a protein tyrosine phosphatase, e.g., CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling, and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685, and 1820) and as allelic forms CD45.1 (Ly-5.1) and CD45.2 (Ly-5.2). The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD45.1 allotype.

Catalog Number: (103264-502)
Supplier: Southern Biotechnology
Description: Alexa Fluor 647 Anti-Mouse IgG1 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.


Catalog Number: (100245-244)
Supplier: Southern Biotechnology
Description: Sheep Anti-FITC-HRP for use in ELISA and immunohistochemistry assays.


Catalog Number: (103265-000)
Supplier: Southern Biotechnology
Description: Alexa Fluor 647 Anti-Goat IgG Fc antibody for use in western blotting, immunohistochemistry, and other fluorescent assays.


Catalog Number: (100244-616)
Supplier: Southern Biotechnology
Description: FITC Anti-Rat IgG2c antibody for use in flow cytometry and immunohistochemistry assays.


Catalog Number: (103259-932)
Supplier: Southern Biotechnology
Description: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.


Catalog Number: (100243-566)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.


Catalog Number: (103265-090)
Supplier: Southern Biotechnology
Description: Purified Donkey F(ab')2 Anti-Rat IgG(H+L) antibody with minimal reactivity to mouse serum proteins for use in ELISA assays.


Supplier: Southern Biotechnology
Description: CD55, also known as decay accelerating factor (DAF), is an 80 kDa GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters.

Catalog Number: (103260-030)
Supplier: Southern Biotechnology
Description: Goat F(ab')2 Anti-Human Lambda-Alexa Fluor 647 antibody for use in flow cytometry and western blotting assays.


Catalog Number: (100246-208)
Supplier: Southern Biotechnology
Description: CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function.


Catalog Number: (103259-958)
Supplier: Southern Biotechnology
Description: NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL/6, NZB, and CE). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.


Catalog Number: (100244-036)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.


Catalog Number: (103264-592)
Supplier: Southern Biotechnology
Description: The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.


Supplier: Southern Biotechnology
Description: Goat Anti-Mouse IgG-PE antibody with minimal reactivity to human proteins for use in flow cytometry and multiplex assays.

Catalog Number: (100244-480)
Supplier: Southern Biotechnology
Description: Goat Anti-Rat IgG-AP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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