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Supplier: Biotium

Description: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®405S is a blue fluorescent dye (Ex/Em 404/431 nm) with superior brightness compared to other blue dyes; it is also compatible with super-resolution imaging by SIM. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Supplier: Biotium

Description: CD31 / PECAM-1 Monoclonal antibody, Clone: JC70/A + C31.3, Host: Mouse, Conjugate: CF640R, Species reactivity: Rabbit, Cynomolgus monkey, Human, Immunogen: Recom CD31 protein (C31.3), Isotype: IgG, Synonyms:EndoCAM, Application: IF, IHC, FC, Size: 100uL

Catalog Number: (75976-732)

Supplier: Biotium

Description: TNF alphA, Monoclonal antibody, Clone: 4C6-H8, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, BSA-free, Immunogen: Recombinant human TNF-alpha, Application: Immunofluorescence, Flow cytometry, Size: 50 uL

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Supplier: Biotium

Description: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87%), as opposed to 76% for anti-HMB45 and 82% for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

Description: Cytokeratin 18 Monoclonal antibody, Clone: KRT18/1190, Host: Mouse, Species reactivity: Human, Rat, Isotype: IgG1, kappa, Conjugate: CF647, Immunogen: Recom human KRT18 protein, Synonyms: K18, Application: IF, IHC, FC, Size: 100uL

Supplier: Biotium

Description: This antibody reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Supplier: Biotium

Description: CD45 / LCA Monoclonal antibody, Clone: 135-4C5, Host: Mouse, Species reactivity: Human, Isotype: IgG2b, kappa, Conjugate: CF660R, Immunogen: Stimulated human leukocytes, Synonyms: B220, CD45R, Application: IF, IHC, FC, Size: 500 uL

Supplier: Biotium

Description: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®594 is a deep red fluorescent dye (Ex/Em 593/614 nm). It yields the brightest conjugates among spectrally similar dyes, and has excellent photostability.

Catalog Number: (75976-356)

Supplier: Biotium

Description: TGF-alpha, Monoclonal antibody, Clone: TGFA/1119, Host: Mouse, Species reactivity: Rabbit, Zebrafish, Human, Isotype: IgG1, kappa, BSA-free, Immunogen: A 10-amino acid synthetic peptide (aa34-43), Synonyms: EGF-like TGF; ETGF, Application: Immunofluorescence, Size: 50 uL

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Catalog Number: (89427-200)

Supplier: Biotium

Description: Antibody Conjugates Prepared From Near-Infrared Cf790 Dye Are Brighter And Have The Best Signal-To-Noise Ratio Compared To Spectrally Similar Dyes; Antibody Has Minimal Cross-Reactivity With Human, Mouse And Rat Serum Proteins

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Supplier: Biotium

Description: Vimentin, Monoclonal antibody, Clone: LN-6, Host: Mouse, Species reactivity: Mouse, Pig, Cow, Cat, Sheep, Rat, Human, Rabbit, Isotype: IgM, Conjugate: purified, Immunogen: Human thymic nuclear extract, Synonyms: VIM, Application: IF, Size: 500uL

Catalog Number: (75891-262)

Supplier: Biotium

Description: This antibody recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1 adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb This antibody recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.

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Catalog Number: (10001-956)

Supplier: Biotium

Description: Llama anti-mouse CF™ dye conjugates are prepared by labeling affinity purified llama anti-mouse IgG (H+L) with a selection of CF™ dyes. CF™ dyes offer combined advantages in brightness, photostability, and specificity compared other fluorescent dyes. CF™594 (Ex/Em 593/614 nm) is a deep-red dye spectrally similar to Alexa Fluor® 594 and Texas Red® dye but yields much brighter protein conjugates due to its high quantum yield and exceptional water solubility. CF™594 also has excellent photostability ideal for demanding applications, such as confocal microscopy and single molecule imaging.

Alexa Fluor® and Texas Red® are registered trademarks of Thermo Fisher Scientific.

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Supplier: Biotium

Description: This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this MAb is mapped between aa120-170. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Supplier: Biotium

Description: This antibody reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Catalog Number: (75970-920)

Supplier: Biotium

Description: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

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