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Supplier: Biotium

Description: This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

Description: This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes; DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®405S is a blue fluorescent dye (Ex/Em 404/431 nm) with superior brightness compared to other blue dyes; it is also compatible with super-resolution imaging by SIM. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Supplier: Biotium

Description: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Supplier: Biotium

Description: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Supplier: Biotium

Description: CD90 Monoclonal antibody, Clone: F15-42-1-5, Host: Mouse, Species reactivity: Human, Monkey, Isotype: IgG1, kappa, Conjugate: CF647, Immunogen: Purified human brain Thy1Unigene 644697, Application: Immunofluorescence, Flow cytometry, Size: 100uL

Supplier: Biotium

Description: This antibody recognizes a protein of 25-26 kDa, identified as the bcl-2 α oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 α oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 α protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Supplier: Biotium

Description: CD43Monoclonal antibody, Clone: DF-T1, Host: Mouse, Conjugate: CF640R, Species reactivity: Human, Isotype: IgG1, kappa, Immunogen: Myeloblastic KG1 cells, Synonyms: GALGP, GPL115, Application: IF, IHC, FC, Size: 500 uL

Supplier: Biotium

Description: PLAP (Placental Alkaline Phosphatase), Monoclonal antibody, Clone: ALP/870, Host: Mouse, Species reactivity: Human, Isotype: IgM, kappa, Conjugate: CF568, Immunogen: Recombinant full-length human ALPP protein, Application: IF, Immunohistology (formalin), Flow, Size: 500uL

Supplier: Biotium

Description: Insulin, Monoclonal antibody, Clone: E2-E3 + 2D11-H5, Host: Mouse, Species reactivity: Mouse, Pig, Cow, Human, Rabbit, Isotype: IgG1, kappa, Conjugate: CF640R, Immunogen: Purified pig insulin, conjugated to KLH, Synonyms: IDDM2, ILPR, IRDN, MODY10, Application: IF, Size: 100uL

Catalog Number: (75957-220)

Supplier: Biotium

Description: Macrophage Specific Antigen, Monoclonal antibody, Clone: LN-5, Host: Mouse, Species reactivity: Human, Isotype: IgM, kappa, BSA-free, Immunogen: Human peripheral blood mononuclear cells, Application: Immunofluorescence, Flow cytometry, Size: 50 uL

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Supplier: Biotium

Description: Cytokeratin 10 Monoclonal antibody, Clone: LH2, Host: Mouse, Species reactivity: Mouse, Human, Isotype: IgG2a, kappa, Conjugate: CF568, Immunogen: Skin extract of a human Psoriasis patient, Synonyms: BCIE, BIE, EHK, Application: IF, IHC, FC, Size: 500 uL

Catalog Number: (75969-932)

Supplier: Biotium

Description: This MAb reacts with a protein of 20-30 kDa, identified as PGP 9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UCHL-1). Initially, PGP 9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP 9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP 9.5 gene is believed to cause a form of Parkinson's disease.

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Supplier: Biotium

Description: Ep-CAM / CD326 Monoclonal antibody, Clone: EGP40/1120, Host: Mouse, Conjugate: CF405S, Species reactivity: Human, Immunogen: Recombinant full-length human EpCAM protein, Isotype: IgG1, kappa, Application: IF, FC, IHC, WB, Size: 100uL

Supplier: Biotium

Description: This antibody recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Supplier: Biotium

Description: TGF-alpha, Monoclonal antibody, Clone: TGFA/1119, Host: Mouse, Species reactivity: Rabbit, Zebrafish, Human, Isotype: IgG1, kappa, Conjugate: purified, Immunogen: A 10-amino acid synthetic peptide (aa34-43), Synonyms: EGF-like TGF; ETGF, Application: IF, Size: 500uL

Supplier: Biotium

Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.