You Searched For: Cryopreservation

Supplier: IXCELLS BIOTECHNOLOGIES USA MS

Description: Peripheral Blood Mononuclear Cells (PBMCs) from healthy donors

Supplier: MP Biomedicals

Description: 2-8 CELLsium™ medium is a cytoprotective, protein free, ready-to-use biosolution lacking dimethyl sulfoxide (DMSO) for the short-term storage of cells or tissue.

Catalog Number: (76035-948)

Supplier: Lifeline Cell Technology

Description: Lifeline® normal Human Oral Keratinocytes (Gingiva), when grown in Lifeline® DermaLife K Medium, provide an ideal serum-free culture model, for the study of microbial-to-epithelial cell interactions, wound healing, toxicology or oral epithelial biology.

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Supplier: VWR International

Description: Designed for freezing samples in all cryopreservation applications, these vials feature an internal thread with washer, external thread. The caps and vials are made from the same batch of materials to ensure equal dilation coefficient, resulting in optimum sealing characteristics.

Catalog Number: (103051-104)

Supplier: Charles River Laboratories Cell Solutions

Description: Plasma is collected from IRB-consented volunteer donors diagnosed with AML.

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Supplier: VWR International

Description: <p>Designed for freezing samples in all cryopreservation applications, these vials feature an internal thread with washer, external thread. The caps and vials are made from the same batch of materials to ensure equal dilation coefficient, resulting in optimum sealing characteristics. </p>

Catalog Number: (103051-106)

Supplier: Charles River Laboratories Cell Solutions

Description: Serum is collected from IRB-consented volunteer donors diagnosed with AML.

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Supplier: MP Biomedicals

Description: Adonitol (ribitol), a pentose alcohol, is metabolised to teicholic acids used in the cell walls of gram positive bacteria. Adonitol is often compared to other cell permeating molecules such as formamide, propanediol, and DMSO as a cryopreservation agent.

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Supplier: StemBioSys

Description: CELLvo™ Ostoeoarthritic Human Chondrocytes (50-70y) are primary, uncultured cells isolated from recently-diseased cadaver cartilage from donors between 50 and 70 years old. Osteoarthritis status is determined by macroscopic inspection using a modified Outerbridge (1960) scale. Cells are isolated by enzymatic digestion prior to cryopreservation.

Supplier: StemBioSys

Description: CELLvo™ Healthy Human Chondrocytes (50-70y) are primary, uncultured cells isolated from recently-diseased cadaver cartilage from donors between 50 and 70 years old. Healthy status is determined by macroscopic inspection of the tissue using a modified outerbridge (1960) scale in order to distinguish healthy joints from those with signs of osteoarthritis. Cells are isolated by enzymatic digestion prior to cryopreservation.

Supplier: MP Biomedicals

Description: Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.
Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.

SDS

Supplier: Adipogen

Description: Potent, cell permeable, selective and ATP-competitive Rho-associated protein kinases inhibitor, including p160ROCK, ROCK-II and PRK2 inhibitor. Tumor cell invasion and metastasis suppressor. Smooth muscle relaxant. Decreases liver fibrosis by hepatic stellate cell growth inhibition. Antinociceptive. Blocks generation of inflammatory cytokines. Stem cell research modulator. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated human embryonic stem (hES) cells without affecting their pluripotency. Increases survival rate of hES cells undergoing cryopreservation.

Catalog Number: (IC08823051)

Supplier: MP Biomedicals

Description: Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis).

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Supplier: MP Biomedicals

Description: Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: MP Biomedicals

Description: Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

Catalog Number: (MSPP-MR1014)

Supplier: CELLULAR ENGINEERING TECHNOLOGIES M

Description: MEDIA CRYOPRESERVATION

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