TrueBlot® Streptavidin Magnetic Beads
- immunoprecipitation (IP) and Co-immunoprecipitate, and protein-protein interaction in pull-down (using biotinylated antibodies)
- capturing single-stranded-biotinylated DNA oligos, for isolation of biotinylated PCR products, isolation of biotin-labeled DNA-protein complexes from cell or tissue extracts
- Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL, and binding capacity is ≥ 60 µg biotinylated IgG/mg of beads
Trueblot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as Streptavidin, and are specifically designed, tested and quality controlled for magnetic purification of biotin-labeled components using manual or automatic platforms. Streptavidin magnetic beads are stable, pre-blocked beads with high binding capacity that provide rapid and efficient biomolecule purification from complex samples. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL, and binding capacity is ≥ 60 µg biotinylated IgG/mg of beads.
Trueblot® Streptavidin magnetic beads can be used for immunoprecipitation (IP) of antigens (using biotinylated antibodies) from a wide variety of sources and to Co-immunoprecipitate interaction complexes using biotinylated antibodies. Streptavidin magnetic beads can also be used for protein-protein interaction in pull-down assays using biotinylated "bait" proteins and for isolation of biotin-labeled DNA-protein complexes from cell or tissue extracts. These beads are also applied for capturing single-stranded-biotinylated DNA oligos and for isolation of biotinylated PCR products.
