NAD(P)H-Glo Detection System, Promega
Supplier: EBRO Electronic GmbHTotal Ratings: 0
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The NAD(P)H-Glo Detection System is a bioluminescent assay that measures the concentration of the reduced forms of nicotinamide adenine dinucleotides, NADH and NADPH, and does not discriminate between them.
- Monitor the Concentration of Reduced Forms of NADH and NADPH
- Highly sensitive and rapid bioluminescent assay
- Easily adapted for high throughput format
The in vitro enzyme-based NAD(P)H-Glo Detection System is a homogeneous, bioluminescent assay that quantitatively monitors the concentration of the reduced forms of nicotinamide adenine dinucleotides, NADH and NADPH, and does not discriminate between them. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation. In the presence of NAD(P)H, a reductase enzyme reduces a proluciferin reductase substrate to form luciferin. Luciferin then is quantified using Ultra-Glo Recombinant Luciferase, and the light signal produced is proportional to the amount of NAD(P)H in the sample. The reductase and luciferase reactions are initiated by adding an equal volume of a single reagent, which contains reductase, proluciferin Reductase Substrate and Ultra-Glo Recombinant Luciferase, to a NAD(P)H-containing sample. The assay is rapid, requiring only a 40- to 60-minute incubation, has a broad linear range and high signal-to background ratio. The assay is well suited to measuring NAD(P)H production or consumption in high-throughput formats.
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