Anti-CCNB1 Mouse Monoclonal Antibody (Biotin) [clone: V92.1]

Supplier: Biotium

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BNCB0680-100 BNCB0680-500

75923-792EA 205.77 USD

75923-792 75927-714

Anti-CCNB1 Mouse Monoclonal Antibody (Biotin) [clone: V92.1]

Antibodies

This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Type: Primary
Antigen: CCNB1
Clonality: Monoclonal
Clone: Clone V92.1
Conjugation: Biotin
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Hamster, Human, Mouse

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