Anti-IVL Mouse Monoclonal Antibody (CF647) [clone: SY5]

Supplier: Biotium
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BNC470678-100 BNC470678-500
75955-176EA 205.77 USD
75955-176 75955-178
Anti-IVL Mouse Monoclonal Antibody (CF647) [clone: SY5]
Antibodies
This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Type: Primary
Antigen: IVL
Clonality: Monoclonal
Clone: Clone SY5
Conjugation: CF647
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Dog, Gorilla, Human, Monkey, Owl, Pig
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