FLUO 3/AM 90
Supplier: Van Der Voort Lucht en Milieu AmsteTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.
Formula:
C₅₁H₅₀Cl₂N₂O₂₃ MW: 1129.85 g/mol Storage Temperature: Freezer |
CAS Number:
121714-22-5 |
Specification Test Results
Source/Host | Synthetic. |
Purity |
|
Appearance/Color | Dark red powder. |
Solubility | Soluble in DMSO. |
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