Nitro blue tetrazolium chloride (NBT) 98 HPLC_ASSAY_METHOD

Supplier: Van Der Voort Lucht en Milieu Amste
Ratings: (No Reviews)

Total Ratings: 0
Avg. Ratings: 0.0 out of 5

Warning

CDX-N0009-M100 CDX-N0009-G001
102990-596EA 33.23 USD
102990-596 102990-598
Nitro blue tetrazolium chloride (NBT) 98 HPLC_ASSAY_METHOD
Nitro blue tetrazolium chloride (NBT)

NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase). Well-known scavenger of superoxide anions. Dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP). This substrate system produces an insoluble NBT diformazan end product that is blue in color and can be observed visually. When used with BCIP, it is suitable for detection of alkaline phosphatase in western blots, for immunohistological staining procedures and for colorimetric indication of bacterial infection in blood samples. Used as a redox indicator for enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels and pentose shunt dehydrogenses. The NBT/BCIP reaction is also used for colorimetric/spectrophotometric activity assays of oxidoreductases. One application is in activity stains in gel electrophoresis, such as with the mitochondrial electron transport chain complexes.

Formula: C₄₀H₃₀Cl₂N₁₀O₆
MW: 817.65 g/mol
Melting Pt: 200
Storage Temperature: Freezer
MDL Number: MFCD00012159
CAS Number: 298-83-9

Order Now

Specification Test Results

Source/Host Synthetic.
Purity >98% (HPLC)
Appearance/Color Yellow powder.
Solubility Soluble in water.


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR