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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Catalog Number: 77506-808
Supplier: SGI - DNA Synthetic Genomics


Description: Quick Blunting Kit - 100 rxns
Catalog Number: 101417-940
Supplier: New England Biolabs (NEB)

Small Business Enterprise


Description: NEB PCR Cloning Kit
Catalog Number: 102877-564
Supplier: New England Biolabs (NEB)

SDS Small Business Enterprise


Description: Quick Blunting Kit - 20 rxns
Catalog Number: 101417-942
Supplier: New England Biolabs (NEB)

Small Business Enterprise


Catalog Number: 77506-810
Supplier: SGI - DNA Synthetic Genomics


Description: Components: T7Select EcoR I/Hind III Vector Arms, T7Select Control Insert, T7 Packaging Extracts, T7Select Packaging Control DNA, BLT5403, BLT5615, and BL21 glycerol stock, T7SelectUP primer, T7SelectDOWN primer, Storage +2degreeC to +8degreeC
Catalog Number: EM70550-3
Supplier: MilliporeSigma

Description: Ampligase* 10X Reaction Buffer, High specificity and stringency permits sensitive detection of SNPs11, igase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA stru
Catalog Number: 76081-596
Supplier: Lucigen


Description: Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensiti
Catalog Number: 76081-602
Supplier: Lucigen


Catalog Number: 97065-204
Supplier: Lucigen


Description: Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Catalog Number: 76081-594
Supplier: Lucigen


Description: Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Catalog Number: 76081-600
Supplier: Lucigen


Description: Ampligase* Thermostable DNA Ligase with Enzyme and Buffer, atalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are s
Catalog Number: 76081-592
Supplier: Lucigen


Description: Ampligase* DNA Ligase Kit, catalyzes NAD-dependent ligation, Contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. Ampligase Enzyme & Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 u
Catalog Number: 76081-604
Supplier: Lucigen


Catalog Number: 97065-200
Supplier: Lucigen


Description: Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensi
Catalog Number: 76081-598
Supplier: Lucigen


Catalog Number: ST200531
Supplier: Agilent Technologies


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