Description: Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Catalog Number: PI22349

Supplier: Invitrogen

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Description: Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

Catalog Number: PI90003

Supplier: Invitrogen

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Description: Pierce™ Control Agarose Resin is underivatized beaded agarose support for pre-clearing protein immunoprecipitation samples.

Catalog Number: PI26150

Supplier: Invitrogen

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Description: Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Catalog Number: PI29988

Supplier: Invitrogen

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Description: Thermo Scientific CarboxyLink Coupling Resin is for covalent immobilization of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

Catalog Number: PI20266

Supplier: Invitrogen

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Description: The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Catalog Number: PI44680

Supplier: Invitrogen

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Description: Pierce™ MMTS (methyl methanethiosulfonate) is a small compound that reversibly blocks cysteines and other sulfhydryl groups, enabling the study of enzyme activation and other protein functions.

Catalog Number: PI23011

Supplier: Invitrogen

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Description: Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

Catalog Number: PI28010

Supplier: Invitrogen

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Description: Thermo Scientific Pierce APTS (3-aminopropyltriethoxysilane) is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules.

Catalog Number: PI80370

Supplier: Invitrogen

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Description: Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) are a ready-to-use mix of recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems.

Catalog Number: PI84786

Supplier: Invitrogen

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Description: The Pierce™ Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Catalog Number: PI88281

Supplier: Invitrogen

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Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Catalog Number: PI22360

Supplier: Invitrogen

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Catalog Number: PI88880

Supplier: Invitrogen

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Description: Pierce™ Protein IgG Plus Orientation Kits uses a crosslinker to covalently attach antibody that is bound to Protein A (or Protein G) agarose resin, thereby creating a reusable affinity column for purification of antigens.

Catalog Number: PI44990

Supplier: Invitrogen

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Description: Pierce™ Anti-HA magnetic beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Catalog Number: PI88837

Supplier: Invitrogen

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Description: Thermo Scientific Pierce TSAT is tris-(succinimidyl) aminotriacetate, a novel trifunctional crosslinker that contains three short spacers arms linked to a central nitrogen atom, each ending in an amine-reactive NHS-ester group.

Catalog Number: PI33063

Supplier: Invitrogen

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