APF ~5 mM 98 HPLC_ASSAY_METHOD

Supplier: SAFE AND SURE NEDERLAND B.V.
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620-075-M001
89152-914EA 768.45 USD
89152-914
APF ~5 mM 98 HPLC_ASSAY_METHOD
APF

Fluorescent probe for hROS detection.


  • Formulation: Pale yellow liquid.


Fluorescent reagent (Ex(max): 490 nm; Em(max): 515 nm) for the detection of highly reactive oxygen species (hROS). Immediately reacts with hROS such as hydroxyl radical, peroxynitrite and hypochlorite, and the fluorescence intensity greatly increases. Use of APF together with HPF also allows for specific detection of hypochlorite (-OCl) to elucidate reliable the roles of -OCl in biological systems such as neutrophils. Peroxynitrite can be detected in distinction from nitric oxide and superoxide since APF does not react with nitric oxide, superoxide and hydrogen peroxide. Moreover, APF is resistant to light-induced autooxidation.


Synonym: Aminophenyl fluorescein, 2-[6-(4'-Amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid.

Formula: C₂₆H₁₇NO₅
MW: 423.4 g/mol
Storage Temperature: Refrigerator
CAS Number: 359010-70-1
EINECS: 200-679-5
UN: 2265
ADR: 3 III

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Specification Test Results

Concentration ~5 mM
Formulation Dissolved in 0.47 mL dimethyl formamide
Purity ≥98% (HPLC)


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