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Antibody and Protein and Production in Suspension CHO and HEK Cells

The shift in therapeutics towards biologics has created a need for mammalian systems that can quickly produce high titer proteins in a consistent, reproducible manner. Transient transfection is an attractive option for early stage biologics development because generation of target proteins in usable quantities is much faster and easier compared to stable cell line generation. Suspension 293 and CHO cells are the cell factories of choice because of their growth characteristics and ability to produce post-translationally modified and active proteins.

For high titer protein and antibody production in suspension CHO and HEK 293 cells, Mirus Bio offers validated products to support this workflow.

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CHOgro® Expression System

The CHOgro® Expression System was developed through systematic optimization of transfection protocol parameters including: cell density, transfection reagent, media formulation and culture temperature. With the CHOgro® Expression System, high protein titers can now be achieved in suspension CHO cells through high density transient transfection. Salient features include:

  • Efficient – Enables high protein titers with simple workflow
  • Convenient – Quick adaptation to CHO cell lineages
  • Optimized – High density growth with minimal cell clumping post transfection
  • Worry-free – No commercial license required; animal origin free

Learn more about how this system was developed

TransIT-PRO® Transfection Reagent & Kit

The TransIT-PRO® Transfection Reagent & Kit uses animal origin free components designed for high and reproducible protein yield in suspension CHO and 293 derived cells. Since it is compatible with varied media formulations, the same media can be used for both transient and stable expression. Use TransIT-PRO® with CHOgro® Expression Media for highest yield in suspension CHO cells. TransIT-PRO® outperforms linear PEI in protein yield, while providing a cost-effective alternative to PEI and other protein production workflows.

  • High Protein Yield – in Suspension CHO and 293 cells, including CHO-S and Expi293F™ cells
  • Compatible with Multiple CHO Media Formulations – Use with CHOgro® Expression Medium to achieve greatest yields
  • Simple Workflow – reproducible protein expression with minimal optimization
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