DuPont+Tychem®+2000

Alcian blue

Description: Em (610nm, Water)

Catalog Number: 97061-364

Supplier: VWR

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L(+)-Cysteine hydrochloride monohydrate, high purity

Catalog Number: 97061-206

Supplier: VWR

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Sodium hydrogen carbonate ACS

Description: Sodium hydrogen carbonate ACS

Catalog Number: 97062-462

Supplier: VWR

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Sodium iodide, Ultrapure

Catalog Number: 97062-448

Supplier: VWR

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Phenol ≥99.0% saturated with buffer pH 4.5

Description: For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

Catalog Number: 97064-716

Supplier: VWR

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Phenol ≥99% saturated solution in water, Biotechnology Grade, buffered

Description: Packaged at pH 6.6, this mixture is provided with a supplemental alkaline buffer that
allows the researcher to easily increase the pH to 7.9 for DNA purification. When mixed with Chloroform:Isoamyl Alcohol (24:1), it makes an optimized reagent for DNA purification. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.

Catalog Number: 97064-702

Supplier: VWR

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Water, Cell Culture Grade low in endotoxins

Description: VWR® Cell culture grade water is extremely low in endotoxin, free of DEPC (Diethyl pyrocarbonate) and other chemical additives. The product is 0.2 um filtered. It is suitable in cell culture applications.

Catalog Number: VWRL0200-1000

Supplier: VWR

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n-Dodecyl-β-D-maltoside ≥99.0%, high purity

Description: Non-ionic - containing no charge (polyoxyethylenes or alkyl glucosides).
Non-ionic detergents are suited for solubilizing membrane proteins without altering biological activity.

Catalog Number: 97063-172

Supplier: VWR

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VWR® HistoChoice® Clearing Agent

Description: Less hazardous replacement for xylene and toluene. Compatible with organic mounting media, clearing agent is designed to work with isopropanol in automated processors. It also offers faster wax dissolution and slower evaporation than xylene with no oily residue. Clearing agent can be recycled by distillation.

Catalog Number: 97060-934

Supplier: VWR

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