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Description: The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin.

Catalog Number: 77393-004

Supplier: CUBE BIOTECH

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Description: Elution of Rho1D4-tagged protein from matching purification beads.

Catalog Number: 77392-434

Supplier: CUBE BIOTECH

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Description: Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Catalog Number: 77392-356

Supplier: CUBE BIOTECH

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Description: Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

Catalog Number: 77392-586

Supplier: CUBE BIOTECH

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Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-55 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-55 is named from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-55 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiencies, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Catalog Number: 77392-560

Supplier: CUBE BIOTECH

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Description: PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups.

Catalog Number: 77393-192

Supplier: CUBE BIOTECH

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Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: 77392-808

Supplier: CUBE BIOTECH

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Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Catalog Number: 77392-484

Supplier: CUBE BIOTECH

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Description: Channelrhodopsin 1 is a 7-transmembrane protein that acts as a light-driven cation channel in eukaryotic green algae.

Catalog Number: 77392-874

Supplier: CUBE BIOTECH

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Description: This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Catalog Number: 77392-694

Supplier: CUBE BIOTECH

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Description: Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

Catalog Number: 77393-248

Supplier: CUBE BIOTECH

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Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: 77392-730

Supplier: CUBE BIOTECH

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Description: HighSpec Rho1D4 agarose XL resin is a suspension of 400 µm agarose beads coupled to an anti-Rho1D4 antibody.

Catalog Number: 77393-342

Supplier: CUBE BIOTECH

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Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: 77392-734

Supplier: CUBE BIOTECH

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Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: 77392-740

Supplier: CUBE BIOTECH

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Description: Membrane scaffold protein, human origin.

Catalog Number: 77392-654

Supplier: CUBE BIOTECH

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