You Searched For: AGILENT TECHNOLOGIES, INC (CSD)

Description: The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Catalog Number: 77392-522

Supplier: CUBE BIOTECH

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Description: Mild, non-ionic detergents such as Tridecylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Catalog Number: 77392-410

Supplier: CUBE BIOTECH

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Description: PureCube amine carboxy agarose MagBeads are your best option to couple biomolecules that carry free-standing Amine (NH₂) groups.

Catalog Number: 77393-362

Supplier: CUBE BIOTECH

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Description: PureCube epoxy activated MagBeads are your best option to couple biomolecules that carry free-standing amine (NH₂) and thiol (SH) groups.

Catalog Number: 77393-200

Supplier: CUBE BIOTECH

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Description: The Ultrasolute family evolved from the first amphipol A8-35 is used for highly efficient solubilization and stabilization of membrane proteins without the use of detergents while preserving their activity. They demonstrate tolerance to moderate concentrations of divalent cations and exhibit no absorption at wavelengths >250 nm.

Catalog Number: 77392-580

Supplier: CUBE BIOTECH

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Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.

Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all.

We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Catalog Number: 77392-544

Supplier: CUBE BIOTECH

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Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-45 - are highly alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-45 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Catalog Number: 77392-532

Supplier: CUBE BIOTECH

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Description: Our <i>E. coli</i> cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Catalog Number: 77392-646

Supplier: CUBE BIOTECH

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Description: PureCube NTA XL (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

Catalog Number: 77393-294

Supplier: CUBE BIOTECH

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Description: PureCube Maleimide MagBeads are your best option to couple biomolecules that carry thiol (SH) groups.

Catalog Number: 77393-232

Supplier: CUBE BIOTECH

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Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Catalog Number: 77392-854

Supplier: CUBE BIOTECH

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Description: Membrane scaffold protein, human origin.

Catalog Number: 77392-684

Supplier: CUBE BIOTECH

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Description: Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection.

Catalog Number: 77392-880

Supplier: CUBE BIOTECH

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Description: The FPLC Cartridge assembly press is an efficient tool that simplifies the packaging process for FPLC columns.

Catalog Number: 77392-466

Supplier: CUBE BIOTECH

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Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: 77392-746

Supplier: CUBE BIOTECH

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Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: 77392-764

Supplier: CUBE BIOTECH

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