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Catalog Number: (PI26180)

Supplier: Invitrogen

Description: Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.

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Catalog Number: (PI21558)

Supplier: Invitrogen

Description: Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls..

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Catalog Number: (PI84868)

Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

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Supplier: Invitrogen

Description: Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

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Supplier: Invitrogen

Description: Protein A Agarose is a standard-capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

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Catalog Number: (PI88844)

Supplier: Invitrogen

Description: Magnetic c-Myc-Tag IP/Co-IP kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

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Supplier: Invitrogen

Description: Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples.

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Catalog Number: (PI78115)

Supplier: Invitrogen

Description: Inclusion Body Solubilization Reagent effectively retrieves recombinant proteins that form insoluble aggregates during bacterial overexpression.

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Catalog Number: (PI20160)

Supplier: Invitrogen

Description: The Thermo Scientific Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

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Supplier: Invitrogen

Description: Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Supplier: Invitrogen

Description: The Pierce™ protease and phosphatase inhibitor tablets contains protease and/or phosphatase inhibitors to provide broad spectrum complete protections to proteins from degradations.

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Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ Spin Cups and Spin Columns are convenient tools for manipulating small volumes of affinity supports (5 to 500µL) for protein purification.

Supplier: Invitrogen

Description: Thermo Scientific Pierce High Capacity Streptavidin Agarose is a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads.

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Catalog Number: (PI45350)

Supplier: Invitrogen

Description: Pierce™ Protein A/G Coated Plate IP Kit uses coated Protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

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