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Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 ml) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive. Able to tolerate centrifuge speeds up to 20000.

Supplier: Invitrogen

Description: <i>Gaussia</i> Luc vectors contain a gene cloned from the copepod, <i>Gaussia princeps</i>. The gene encodes a naturally-secreted bioluminescent <i>Gaussia</i> luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Supplier: Invitrogen

Description: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

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Supplier: Invitrogen

Description: Purified (unconjugated) Thermo Scientific Pierce™ Recombinant Protein G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Catalog Number: (PI17892)

Supplier: Invitrogen

Description: Thermo Scientific Pierce EDTA is useful as a chelator of alkaline earth metals, as well as iron, copper and zinc in a variety of laboratory methods.

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Catalog Number: (PI20278)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Coomassie Brilliant Blue R-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

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Supplier: Invitrogen

Description: Thermo Scientific Halt Protease Inhibitor Cocktail (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction from animal and plant tissues, cultured cells,
yeast or bacteria.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human <i>in vitro</i> expression systems and bacterial and mammalian cell lysates.

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Supplier: Invitrogen

Description: Thermo Scientific NeutrAvidin coated plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

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Supplier: Invitrogen

Description: Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

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Catalog Number: (PI88434)

Supplier: Invitrogen

Description: Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

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Supplier: Invitrogen

Description: SuperSignal™ ELISA Pico chemiluminescent substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer based assays.

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Catalog Number: (PI22363)

Supplier: Invitrogen

Description: Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).

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Catalog Number: (PI34002)

Supplier: Invitrogen

Description: The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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